Mousemonoclonalantibody
Theantigenisaratalpha-CGRP.
TheantibodyisproteinGpurified.
CalcitoninGeneRelatedPeptide[CGRP]isa37aminoacidpeptideandisthemostpotentendogenousvasodilatorcurrentlyknown(Brainetal.,1985).CGRPisprimarilyproducedinnervoustissue,however,itsreceptorsareexpressedthroughoutthebody.CGRPisalsostronglyimplicatedinthevasodilatoryeffectofendogenouscannABInoidanandamideinthebrain.Thiseffectwasfoundtobeantagonizedbycapsazepine.CGRPisalsocurrentlyamajortargetofresearchinregardstofactorseffectingtheonsetofmigraineheadaches(EdvinssonL.,2003).CGRPantibodieshavebeenusedsuccessfullyinimmunoneutralization(Wongetal.,1993)
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternblots.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1yearafterdateofreceipt.Avoidfreeze/thawcycles.
BrainSD,WilliamsTJ,TippinsJR,MorrisHR,MacIntyreI(1985)Calcitoningenerelatedpeptideisapotentvasodilator.Nature313(5997):54-6
EdvinssonL(2003)Newtherapeutictargetinprimaryheadaches—blockingtheCGRPreceptor.ExpertOpinTherTargets.7(3):377-83
WongHC,Tache’Y,LloudKCK,YangH,SterniniC,HolzerP,WalshJH(1993)Monoclonalantibodytoratalpha-CGRP:production,characterization,andinvivoimmunoneutralizationactivity.Hybridoma.12:93-106.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。