Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser783ofGABAB.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Gamma-aminobutyricacid(GABA)istheprimaryinhibitoryneurotransmitterinthecentralnervoussystem.TherearetwomajorclassesofGABAreceptors:theGABAAandtheGABABsubtypeofreceptors.GABABreceptorsareheterodimericGprotein-coupledreceptorsthatmediateslowsynapticinhibitioninthecentralnervoussystem.IthasrecentlybeendemonstratedthatAMPKbindsdirectlytoGABABreceptorsandphosphorylatesS783inthecytoplasmictailoftheR2subunitandthatS783playsacriticalroleinenhancingneuronalsurvivalafterischemiaasphosphorylationofS783isevidentinmanybrainregionsandisincreaseddramaticallyafterischemicinjurytothebrain(Kuramotoetal.,2007).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Kim,S.H.,Park,S.,Yu,H.S.,Ko,K.H.,Park,H.G.andKim,Y.S.,2017.TheantipsychoticagentclozapineinducesautophagyviatheAMPK-ULK1-Beclin1signalingpathwayintheratfrontalcortex.ProgressinNeuro-PsychopharmacologyandBiologicalPsychiatry.PMID:29079139
deVelasco,E.M.F.,Hearing,M.,Xia,Z.,Victoria,N.C.,Luján,R.,&Wickman,K.(2015).SexdifferencesinGABABR-GIRKsignalinginlayer5/6pyramidalneuronsofthemouseprelimbiccortex.Neuropharmacology.Aug;95:353-60.PMID:25843643
TerunumaM,HaydonPG,PangalosMN,MossSJ.(2015).PurinergicreceptoractivationfacilitatesastrocyticGABABreceptorcalciumsignalling.Neuropharmacology88:74-81.PMID:25261019
TerunumaM,Revilla-SanchezR,QuadrosIM,DengQ,DeebTZ,LumbM,SicinskiP,HaydonPG,PangalosMN,MossSJ.(2014).PostsynapticGABABreceptoractivityregulatesexcitatoryneuronalarchitectureandspatialmemory.JNeurosci.34:804-816.PMID:24431439
TerunumaM,VargasKJ,WilkinsME,RamírezOA,Jaureguiberry-BravoM,PangalosMN,SmartTG,MossSJ,CouveA.(2010).ProlongedactivationofNMDAreceptorspromotesdephosphorylationandalterspostendocyticsortingofGABABreceptors.ProcNatlAcadSciUSA107:13918-13923.PMID:20643948
KuramotoN,WilkinsME,FairfaxBP,Revilla-SanchezR,TerunumaM,TamakiK,IemataM,WarrenN,CouveA,CalverA,HorvathZ,FreemanK,CarlingD,HuangL,GonzalesC,CooperE,SmartTG,PangalosMN,MossSJ(2007)Phospho-dependentfunctionalmodulationofGABA(B)receptorsbythemetabolicsensorAMP-dependentproteinkinase.Neuron53:233-247.PMID:17224405
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。