Rabbitpolyclonalantibody
TheantigenisapeptidecorrespondingtoaminoacidresiduesfromtheC-terminalregionofmouseribosomalS6kinase2,conjugatedtokeyholelimpethemocyanin(KLH).
Theantibodyis preparedfromrabbitserumbyaffinitypurificationviachromatographyonanaffinitycolumnmadewiththeC-terminalpeptideusedasantigen.
Thep90ribosomalS6kinases(RSK)1–4aredownstreammembersoftheextracellularsignal-regulatedkinase(ERK)/MAPKcascade.ThelossofRSK2activityinhumansleadstoCoffin–Lowrysyndrome,whichischaracterizedbymentalretardationandgrowthdeficits(HanauerandYoung,2002).RecentworksuggeststhatRSK2exertsatonicregulationonG-proteincoupledsignaling(Sheffleretal.,2006).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
HanauerA,YoungID(2002)Coffin-Lowrysyndrome:clinicalandmolecularfeatures.JMedGenet39:705-713.
ShefflerDJ,KroezeWK,GarciaBG,DeutchAY,HufeisenSJ,LeahyP,BruningJC,RothBL(2006)p90ribosomalS6kinase2exertsatonicbrakeonGprotein-coupledreceptorsignaling.ProcNatlAcadSci(USA)103:4717-4722.
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