Sheeppolyclonalantibody
PooledSerumTheantigenisapeptidefromintracellularC-terminalregionofhumanVMAT2,conjugatedtokeyholelimpethemocyanin(KLH).
Theantibodyispreparedfromsheepserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
Vesicularneurotransmittertransporterssequesterthetransmittersintosynapticvesicles(Ericksonetal.,1996).Thevesicularmonoaminetransporter2(VMAT2)isresponsIBLeforcatecholamineandSEROtoninstorageincentralsynapses.AntibodiesspecificforVMAThavebeenusedtomonitorexpressionofthetransporterduringdevelopmentandinagingandcanbeeffectivelyusedasaMarkerformonoamineterminals(Haycocketal.,2003;Witkovskyetal.,2005).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternblots.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
Ivanova,E.,Yee,C.W.,&Sagdullaev,B.T.(2015).Disruptionindopaminergicinnervationduringphotoreceptordegeneration.JournalofComparativeNeurology.Sep10.PMID:26356010
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。