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主营:神经科学领域的蛋白及抗体
℡ 4000-520-616
℡ 4000-520-616
PhosphoSolutions/Tyrosine Hydroxylase (Ser31) Antibody/p1580-31/100 µl
产品编号:p1580-31
市  场 价:¥4574.70
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$345.00
品      牌: PhosphoSolutions
公      司:PhosphoSolutions
公司分类:
PhosphoSolutions/Tyrosine Hydroxylase (Ser31) Antibody/p1580-31/100 µl
商品介绍

Rabbitpolyclonalantibody

PooledSerum
Size:
100µl
Formulation:
AffinityPurifiedfromPooledSerum
Specificity:
Rat,Mouse,Non-humanprimate
Applications:
WB1:1,000IF1:1,000IHC1:1,000
Species:
Rabbit
GeneName:
TH
MolecularReference:
~60kDa
CiteThisAntibody:
PhosphoSolutionsCat#p1580-31,RRID:AB_2492278
Antigen/Purification:ExpandCollapse

Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingphospho-Ser31ofrattyrosinehydroxylase(TH).

Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.

BIOLOGicalSignificance:ExpandCollapse

Tyrosinehydroxylase(TH)istherate-limitingenzymeinthesynthesisofthecatecholaminesdopamineandnorepinephrine.THantibodiescanthereforebeusedasMarkersfordopaminergicandnoradrenergicneuronsinavarietyofapplicationsincludingdepression,schizophrenia,Parkinson’sdiseaseanddrugabuse(Kishetal.,2001;Zhuetal.,2000;Zhuetal.,1999).THantibodiescanalsobeusedtoexplorebasicmechanismsofdopamineandnorepinephrinesignaling(Witkovskyetal.,2000;Salvatoreetal.,2001;Dunkleyetal.,2004).TheactivityofTHisalsoregulatedbyphosphorylation(Haycocketal.,1982;Haycocketal.,1992;Jedynaketal.,2002).Phospho-specificantibodiesforthephosphorylationsitesonTHcanbeusedtogreateffectinstudyingthisregulationandinidentifyingthecellsinwhichTHphosphorylationoccurs.

Storage

100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

ProductSpecificReferences

Jorge-Finnigan,A.,Kleppe,R.,Jung-KC,K.,Ying,M.,Marie,M.,Rios-Mondragon,I.,Salvatore,M.F.,Saraste,J.andMartinez,A.,2017.Phosphorylationatserine31targetstyrosinehydroxylasetovesiclesfortransportalongmicrotubules.JournalofBiologicalChemistry,pp.jbc-M116.PMID:28637871

Bonito-Oliva,Alessandra,GiadaSpigolon,andGilbertoFisone.(2015)“RegulationofPre-andPostsynapticProteinPhosphorylationbyDopamineD2Receptors.”DopamineReceptorTechnologies.SpringerNewYork,2015.79-100.

Izumi,Y.,Ezumi,M.,Takada-Takatori,Y.,Akaike,A.,&Kume,T.(2014).Endogenousdopamineisinvolvedintheherbicideparaquat-induceddopaminergiccelldeath.ToxicologicalSciences,139(2),466-478.PMID:24743698

Calipari,E.S.,Ferris,M.J.,Melchior,J.R.,Bermejo,K.,Salahpour,A.,Roberts,D.,&Jones,S.R.(2014).Methylphenidateandcocaineself‐admiNISTrationproducedistinctdopamineterminalalterations.Addictionbiology,19(2),145-155.PMID:22458761

JedynakJP,AliSF,HaycockJW,HopeBT(2002)Acuteadministrationofcocaineregulatesthephosphorylationofserine-19,-31and-40intyrosinehydroxylase.JNeuRochem82:382-388.PMID:12124439

SalvatoreMF,WaymireJC,HaycockJW(2001)Depolarization-stimulatedcatecholaminebiosynthesis:involvementofproteinkinasesandtyrosinehydroxylasephosphorylationsitesinsitu.JNeurochem79:349-360.PMID:11677263

WitkovskyP,GabrielR,HaycockJW,MellerE(2000)Influenceoflightandneuralcircuitryontyrosinehydroxylasephosphorylationintheratretina.JChemNeuroanat19:105-116.PMID:10936746

品牌介绍

PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。

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