Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser368ofratconnexin43.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Gapjunctionalintercellularcommunicationisthoughttoplayakeyroleindevelopmentandmayalsobeinvolvedinepilepsy(Aronicaetal.,2001).Connexin43formsgap-junctionalchannelsandregulatesthepermeABIlityofthesegapjunctionstosmallorganicmolecules.Permeabilityofconnexin43isknowntoberegulatedbyphosphorylationatSer368byproteinkinaseC(Yogoetal.,2002;Baoetal.,2004a).PhosphorylationofSer368 byPKCinducesaconformationalchangeofconnexin43thatresultsinadecreaseingapjunctionpermeability(Baoetal.,2004b).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
AronicaE,GorterJA,JansenGH,LeenstraS,YankayaB,TroostD(2001)Expressionofconnexin43andconnexin32gap-junctionproteinsinepilepsy-associatedbraintumorsandintheperilesionalepilepticcortex.ActaNeuropathol(Berl)101:449-459.
BaoX,AltenbergGA,ReussL(2004a)Mechanismofregulationofthegapjunctionproteinconnexin43byproteinkinaseC-mediatedphosphorylation.AmJPhysiolCellPhysiol286:C647-C654.
BaoX,ReussL,AltenbergGA(2004b)Regulationofpurifiedandreconstitutedconnexin43hemichannelsbyproteinkinaseC-mediatedphosphorylationofSerine368.JBiolChem279:20058-20066.
YogoK,OgawaT,AkiyamaM,IshidaN,TakeyaT(2002)IdentificationandfunctionalanalysisofnovelphosphorylationsitesinCx43inratprimarygranulosacells.FEBSLett531:132-136.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。