RabbitPolyclonalAntibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephosphoTyr153ofhumanCK1Mt.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephosphopeptideaffinitycolumns.
Creatinekinase(CK1)playsanimportantroletransferringphosphategroupsfromphosphocreatinetoADPinthecytosoloftissueshavinghigh,fluctuatingenergydemandslikeskeletalmuscle,heart,andbrain. MitochondrialCK1,CK1Mt,hastwoisoforms,sarcomericCK1MtandubiquitousCK1Mt. CK1MtislocalizedbetweentheMIM(mitochondrialinnermembrane)andMOM(mitochondrialoutermembrane)andboundtothecardiolipin-richinnerleaflet(Mulleretal.,1985)andalongthecristaemembranes(Wegmannetal.,1991). CK1Mtexistsintwoforms;ahomodimerandanoctamerconsistingoffourhomodimers. TheCK1MtoctamerformspermanentcontactsitesandmaintainsacomplexstructureincludingporinandANT(adeninenucleotidetranslocase)withinMIMandMOM(Speeretal,2005).Therehavebeenseveralphospho-serine,threonine,andtyrosinesitesidentifiedwithinCK1Mt,theroleofeachonehasyettobedetermined.
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Müller,M.,Moser,R.,Cheneval,D.,Carafoli,E.(1985)CardiolipinistheMembraneReceptorforMitochondrialCreatinePhosphokinase.J.Biol.Chem.260,3839-3843.
Wegmann,G.,Huber,R.,Zanolla,E.,Eppenberger,H.M.,Wallimann,T.(1991)DifferentialExpressionandLocalizationofBrain-TypeandMitochondrialCreatineKinaseIsoenzymesDuringDevelopmentoftheChickenRetina:Mi-CKasaMarkerforDifferentiationofPhotoreceptorCells.Differentiation46,77-87.
SpeerO,BäckN,BuerklenT,BrdiczkaD,KoretskyA,WallimanT,ErikssonO(2005)OctamericMitochondrialCreatineKinaseInducesandStABIlizesContactSitesBetweentheInnerandOuterMembrane.Biochem.J.385,445-450.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。