Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser394ofhumanHDAC2.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
HistoneDeacetylase2(HDAC2)ispartofafamilyofhistonedeacetylasesthatareresponsIBLefordeacetylationoflysineresiduesinthehistonecore.HDAC2isclassifiedasaclassIhistonedeacetylaseandisubiquitouslyexpressedthroughoutthebody(Keeetal,2008).IthasbeenshownthatHDAC2playsanimportantroleincardiachypertrophy(Eometal,2011).Phosphorylationofser394isresponsibleforthehypertrophy-associatedactivationofHDAC2,whereasintrinsicbasalactivityismaintainedbyphosphorylationofSer422andSer424(EOMetal,2011).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
KeeHJ,EomGH,JoungH,ShinS,KimJRChoYK,ChoeN,SimBW,JoD,JeongMH,KimKK,SeoJS,KookH(2008)Activationofhistonedeacetylase2byinducibleheatshockprotein70incardiachypertrophy.CirRes2008Nov21;103(11):1259-69.
EomGH,ChoYK,KoJH,ShinS,ChoeN,KimY,JoungH,KimHS,NamKI,KeeHJ,KookH(2011)CaseinKinase-2α1InducesHypertrophicResposebyPhosphorylationofHistoneDeacetylase2S394anditsactivationintheHeartClinicalPerspective.CirculationMay16,2011;123:2392-2403.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。