RabbitPolyclonalAntibody
PooledSerumPhosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr53ofmouseNCC.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephosphopeptideaffinitycolumns.
Thethiazide-sensitivesodiumchloridecotransporter,NCC,isthemajorNaCltransportproteininthedistalconvolutedtubule(DCT)andplaysanimportantroleinmaintainingbloodpressure(Rosenbaeketal.,2014,Fengetal.,2015).PhosphorylationofNCCatThr-53,Thr-58,andSer-71isanessentialmediatorofNCCfunction(Rosenbaeketal.,2014).NCCisconstitutivelycycledtotheplasmamembrane,anduponstimulation,itcanbephosphorylatedtobothincreaseNCCactivityanddecreaseNCCendocytosis,togetherincreasingNaCltransportintheDCT(Fengetal.,2015).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Palygin,O.,Levchenko,V.,Ilatovskaya,D.V.,Pavlov,T.S.,Pochynyuk,M.,Jacob,H.J.,Geurts,A.M.,Hodges,M.R.andStaruschenko,A.,2017.EssentialroleofKir5.1channelsinrenalsalthandlingandbloodpressurecontrol.JCIInsight,2(18).PMID:28931751
TerkerAS,YarbroughB,FerdausMZ,LazelleRA,ErspamerKJ,MeermeierNP,ParkHJ,McCormickJA,YangCL,EllisonD3.(2016)DirectandIndirectMineralocorticoidEffectsDetermineDistalSaltTransport.JAmSocNephrol.(8):2436-45. PMID:26712527
TerkerAS,ZhangC,McCormickJA,LazelleRA,ZhangC,MeermeierNP,SilerD,ParkHJ,FuY,CohenDM,WeinsteinAM,WangWH,YangCL,EllisonDH.(2015)Potassiummodulateselectrolytebalanceandbloodpressurethrougheffectsondistalcellvoltageandchloride.CellMetab.(1):39-50.PMID:25565204
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。