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主营:神经科学领域的蛋白及抗体
℡ 4000-520-616
℡ 4000-520-616
PhosphoSolutions/DARPP 32 (Thr34) Antibody/p1025-34/100 µl
产品编号:p1025-34
市  场 价:¥4574.70
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$345.00
品      牌: PhosphoSolutions
公      司:PhosphoSolutions
公司分类:
PhosphoSolutions/DARPP 32 (Thr34) Antibody/p1025-34/100 µl
商品介绍

Rabbitpolyclonalantibody

PooledSerum
Size:
100µl
Formulation:
AffinityPurifiedfromPooledSerum
Specificity:
Rat,Mouse,Human,Bovine,Chicken,Non-humanprimate,Canine
Applications:
WB1:1,000
Species:
Rabbit
GeneName:
PPP1R1B
MolecularReference:
~32kDa
CiteThisAntibody:
PhosphoSolutionsCat#p1025-34,RRID:AB_2492068
Antigen/Purification:ExpandCollapse

Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr34ofratDARPP-32.

Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.

BIOLOGicalSignificance:ExpandCollapse

DARPP-32isadopamine(DA)andcAMP-regulated~32kphosphoproteinthatisassociatedwithdopaminoceptiveneurons(Fienbergetal.,1998).TheproteininhibitsproteinphosphataseIwhenitisphosphorylatedonThr34.Incontrast,whenDARPP-32isphosphorylatedonThr75theproteinactsasaninhibitorofPKA(Bibbetal.,1999).PhosphorylationofDARPP-32isthoughttoplayacriticalroleintheregulationofdopaminergicneurotransmission.Inaddition,theactivityofDARPP-32isalsothoughttoplayimportantrolesintheactionsofalcohol,caffeineandProzac®(Maldveetal.,2002;Lindskogetal.,2002;Svenningssonetal.,2002).

Storage

100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

ProductSpecificReferences

Bonito-Oliva,A.,Södersten,E.,Spigolon,G.,Hu,X.,Hellysaz,A.,Falconi,A.,Gomes,A.L.,Broberger,C.,Hansen,K.,&Fisone,G.(2016).DifferentialregulationofthephosphorylationofTrimethyl-lysine27histoneH3atserine28indistinctpopulationsofstriatalprojectionneurons.Neuropharmacology,107,89-99. PMID:26947946

Beaulieu,J.M.(2015).Invivoveritas,thenextfrontierforfunctionallyselectiveGPCRligands.Methods.Aug28.PMID:26320830

Bonito-Oliva,A.,DuPont,C.,Madjid,N.,Ögren,S.O.,&Fisone,G.(2015).Involvementofthestriatalmediumspinyneuronsofthedirectpathwayinthemotorstimulanteffectsofphencyclidine.Theinternationaljournalofneuropsychopharmacology/(CINP).PMID:26657176

Del’Guidice,T.,&Beaulieu,J.M.(2015).SelectivedisruptionofdopamineD2-receptors/beta-arrestin2signalingbymoodstABIlizers.JournalofReceptorsandSignalTransduction,1-9.Jun;35(3):224-32.PMID:26459714

ThomasDel’Guidice,CamilleLatapy,AntonioRampino,JivanKhlghatyan,MorganeLemasson,BarbaraGelao,TizianaQuarto,GiuseppeRizzo,AnnieBarbeau,ClaudeLamarre,AlessandroBertolino,GiuseppeBlasi,andJean-MartinBeaulieu(2015)FXR1PisaGSK3βsubstrateregulatingmoodandemotionprocessing.PNAS,Aug18;112(33):E4610-9.PMID:26240334

DanielaMarazziti,ChiaraDiPietro,SilviaMandillo,ELISAbettaGolini,RafaeleMatteoni,andGlaucoP.Tocchini-Valentini(2011)AbsenceoftheGPR37/PAELreceptorimpairsstriatalAktandERK2phosphorylation,FosBexpression,andconditionedplacepreferencetoamphetamineandcocaine.FASEBJ,25:2071–2081.PMID:21372109

RajaniMaiya,YanZhou,ErinH.Norris,MaryJeanneKreek,andSidneyStrickland(2009)Tissueplasminogenactivatormodulatesthecellularandbehavioralresponsetococaine.PNAS,106:1983–1988.PMID:19181855

MichelleNiculescu,ShaneA.Perrine,JonathanS.Miller,MichelleE.Ehrlich,andEllenM.Unterwald(2008)Trk:ANeuromodulatorofAge-SpecificBehavioralandNeuRochemicalResponsestoCocaineinMice.J.Neurosci.,Jan2008;28:1198–1207.PMID:18234897

Jean-MartinBeaulieu,TatyanaD.Sotnikova,RaulR.Gainetdinov,andMarcG.Caron(2006)ParadoxicalStriatalCellularSignalingResponsestoPsychostimulantsinHyperactiveMice.J.Biol.Chem.,Oct2006;281:32072–32080.PMID:16954211

品牌介绍

PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。

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