Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Affinitypurified
- Specificity:
- Rat,Mouse,Human,Bovine,Chicken,Non-humanprimate,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- PPP1R1B
- MolecularReference:
- ~32kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1025-75,RRID:AB_2492069
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr75ofratDARPP-32.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
DARPP-32isadopamine(DA)andcAMP-regulated~32kDaphosphoproteinthatisassociatedwithdopaminoceptiveneurons(Fienbergetal.,1998).TheproteininhibitsproteinphosphataseIwhenitisphosphorylatedonThr34.Incontrast,whenDARPP-32isphosphorylatedonThr75theproteinactsasaninhibitorofPKA(Bibbetal.,1999).PhosphorylationofDARPP-32isthoughttoplayacriticalroleintheregulationofdopaminergicneurotransmission.Inaddition,theactivityofDARPP-32isalsothoughttoplayimportantrolesintheactionsofalcohol,caffeineandProzac®(Maldveetal.,2002;Lindskogetal.,2002;Svenningssonetal.,2002).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
MichelleNiculescu,ShaneA.Perrine,JonathanS.Miller,MichelleE.Ehrlich,andEllenM.Unterwald(2008)Trk:ANeuromodulatorofAge-SpecificBehavioralandNeuRochemicalResponsestoCocaineinMice.J.Neurosci.,Jan2008;28:1198–1207. PMID:18234897
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik，他们在 Paul Greengard 博士的实验室共同工作，Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区，是一支敬业、友好、勤奋的科学家团队，致力于为您提供最优质的抗体和业内客户支持。
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