Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Bovine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- DDC
- MolecularReference:
- ~55kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#417-DDC,RRID:AB_2492072
Antigen/Purification:Collapse
TheantigenisanSDSdenatured,recombinantbovinearomaticDOPAdecarboxylase(DDC)expressedinE.coliandpurifiedfrominclusionbodies.
TheantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusinganAminOlink®Pluscolumnmatrixtowhichpurified,recombinantbovineDDCwascoupled.
BIOLOGicalSignificance:Collapse
DOPAdecarboxylase(aromaticL-aminoaciddecarboxylase,AADC;DDC)catalyzesthesecondreactioninthebiosynthesisofcatecholaminesandSEROtonin(WaymireandHaycock,2002;Berryetal.,1996;Haycocketal.,2003).Itisalsoinvolvedinthebiosynthesisoftraceamines.DDCantibodiescanthereforebeusedasMarkersfordopaminergic,noradrenergicandserotonergicneuronsinavarietyofapplicationsincludingdepression,schizophrenia,Parkinson’sdiseaseanddrugabuse(Kishetal.,2001;Zhuetal.,2000;Zhuetal.,1999).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
HaycockJW,BeckerL,AngL,FurukawaY,HornykiewiczO,KishSJ(2003)Markeddisparitybetweenage-relatedchangesindopamineandotherpresynapticdopaminergicmarkersinhumanstriatum.JNeuRochem87:574-585.PMID:14535941
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik，他们在 Paul Greengard 博士的实验室共同工作，Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区，是一支敬业、友好、勤奋的科学家团队，致力于为您提供最优质的抗体和业内客户支持。
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