Rabbitpolyclonalantibody
PooledSerumTheantigenisa phosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr53ofratDAT.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Thedopaminetransporter(DAT)isresponsIBLeforthereaccumulationofdopamineafterithasbeenreleased.DATantibodiesandantibodiesforotherMarkersofcatecholaminebiosynthesisarewidelyusedasmarkersfordopaminergicandnoradrenergicneuronsinavarietyofapplicationsincludingdepression,schizophrenia,Parkinson’sdiseaseanddrugabuse(Kishetal.,2001;Zhuetal.,2000;Zhuetal.,1999).LevelsofDATproteinexpressionarealteredbychronicdrugadmiNISTration(Wilsonetal.,1996).IthasbeenshownthatphosphorylationatThr53directlyaffectsdopamineinfluxandamphetamine-stimulatedsubstrateefflux,indicatingthattheThr53residueplaysamajorroleintransportactivity(Fosteretal.,2012).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Sase,A.,Aher,Y.,Saroja,S.R.,Ganesan,M.K.,Sase,S.,Holy,M.,Hoger,H.,Bakulev,V.,Ecker,G.,Langer,T.,Sitte,H.H.,Leban,J.,&Lubec,G.(2015).AheterocycliccompoundCE-103inhibitsdopaminereuptakeandmodulatesdopaminetransporteranddopamineD1-D3containingreceptorcomplexes.Neuropharmacology.Sept22pii:S0028-3908(15)300046-0.PMID:26407764
JamesD.Foster,Jae-WonYang,AmyE.Moritz,SathyavathiChallaSivaKanaka,MargaretA.Smith,MarionHoly,KyleWilebski,HaraldH.Sitte,andRoxanneA.Vaughan(2012)DopamineTransporterPhosphorylationSiteThreonine53RegulatesSubstrateReuptakeandAmphetamine-stimulatedEfflux.JournalofBiologicalChemistry287(35):29702-29712.PMID:22722938
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。