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主营:神经科学领域的蛋白及抗体
℡ 4000-520-616
℡ 4000-520-616
PhosphoSolutions/Dopamine Transporter (DAT) (Thr53) Antibody/p435-53/100 µl
产品编号:p435-53
市  场 价:¥4574.70
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$345.00
品      牌: PhosphoSolutions
公      司:PhosphoSolutions
公司分类:
PhosphoSolutions/Dopamine Transporter (DAT) (Thr53) Antibody/p435-53/100 µl
商品介绍

Rabbitpolyclonalantibody

PooledSerum
Size:
100µl
Formulation:
AffinityPurifiedfromPooledSerum
Specificity:
Rat
Applications:
WB1:1,000
Species:
Rabbit
GeneName:
SLC6A3
MolecularReference:
~55kDa
CiteThisAntibody:
PhosphoSolutionsCat#p435-53,RRID:AB_2492078
Antigen/Purification:ExpandCollapse

Theantigenisa phosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr53ofratDAT.

Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.

 

BIOLOGicalSignificance:ExpandCollapse

Thedopaminetransporter(DAT)isresponsIBLeforthereaccumulationofdopamineafterithasbeenreleased.DATantibodiesandantibodiesforotherMarkersofcatecholaminebiosynthesisarewidelyusedasmarkersfordopaminergicandnoradrenergicneuronsinavarietyofapplicationsincludingdepression,schizophrenia,Parkinson’sdiseaseanddrugabuse(Kishetal.,2001;Zhuetal.,2000;Zhuetal.,1999).LevelsofDATproteinexpressionarealteredbychronicdrugadmiNISTration(Wilsonetal.,1996).IthasbeenshownthatphosphorylationatThr53directlyaffectsdopamineinfluxandamphetamine-stimulatedsubstrateefflux,indicatingthattheThr53residueplaysamajorroleintransportactivity(Fosteretal.,2012).

Storage

100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

ProductSpecificReferences

Sase,A.,Aher,Y.,Saroja,S.R.,Ganesan,M.K.,Sase,S.,Holy,M.,Hoger,H.,Bakulev,V.,Ecker,G.,Langer,T.,Sitte,H.H.,Leban,J.,&Lubec,G.(2015).AheterocycliccompoundCE-103inhibitsdopaminereuptakeandmodulatesdopaminetransporteranddopamineD1-D3containingreceptorcomplexes.Neuropharmacology.Sept22pii:S0028-3908(15)300046-0.PMID:26407764

JamesD.Foster,Jae-WonYang,AmyE.Moritz,SathyavathiChallaSivaKanaka,MargaretA.Smith,MarionHoly,KyleWilebski,HaraldH.Sitte,andRoxanneA.Vaughan(2012)DopamineTransporterPhosphorylationSiteThreonine53RegulatesSubstrateReuptakeandAmphetamine-stimulatedEfflux.JournalofBiologicalChemistry287(35):29702-29712.PMID:22722938

品牌介绍

PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。

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