Rabbitpolyclonalantibody
PooledSerumTheantigenisapeptidefromtheintracellularC-terminusregionofhumandopaminetransporter(DAT),conjugatedtokeyholelimpethemocyanin(KLH).
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationusingaSulfOlink®columnmatrixtowhichthepeptideimmunogenwascoupled.
Thedopaminetransporter(DAT)isresponsIBLeforthereaccumulationofdopamineafterithasbeenreleased.DATantibodiesandantibodiesforotherMarkersofcatecholaminebiosynthesisarewidelyusedasmarkersfordopaminergicandnoradrenergicneuronsinavarietyofapplicationsincludingdepression,schizophrenia,Parkinson’sdiseaseanddrugabuse(Kishetal.,2001;Zhuetal.,2000;Zhuetal.,1999).LevelsofDATproteinexpressionarealteredbychronicdrugadmiNISTration(Wilsonetal.,1996).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
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KishSJ,KalasinskyKS,DerkachP,SchmunkGA,GuttmanM,AngL,AdamsV,FurukawaY,HaycockJW(2001)StriataldopaminergicandSEROtonergicmarkersinhumanheroinusers.Neuropsychopharmacology24:561-567.PMID:11282256
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。