Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser129ofalphasynuclein.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Alpha-synucleinisapresynapticneuronalproteinthatisthoughttobeinvolvedintheformationofSNAREcomplexes.Mostsignificantly,aggregatedalphasynucleinisoneofthemajorcomponentsfoundintheLewybodiesthatoccurinParkinson’sdisease(PD)andotherneurodegenerativedisorders(Okochietal.,2000).Mutationofthealpha-synucleingeneisassociatedwithfamilialformsofPD(Newmannetal.,2002).Recentevidencesuggeststhatphosphorylationofalpha-synucleinatSer129playsaroleintheformationofinclusionsinPD(Smithetal.,2005;Masliahetal.,2005).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
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NeumannM,KahlePJ,GiassonBI,OzmenL,BorroniE,SpoorenW,MullerV,OdoyS,FujiwaraH,HasegawaM,IwatsuboT,TrojanowskiJQ,KretzschmarHA,HaassC(2002)Misfoldedproteinasekresistanthyperphosphorylatedalpha-synucleininagedtransgenicmicewithlocomotordeteriorationandinhumanalphasynucleinopathies.JClinInvestNov;110(10):1403-5.
SmithWW,MargolisRL,LiX,TroncosoJC,LeeMK,DawsonVL,DawsonTM,IwatsuboT,RossCA(2005)Alpha-synucleinphosphorylationenhanceseosinophiliccytoplasmicinclusionformationinSH-SY5YCells.JNeurosciJune8;25(23):5544-5552.
MasliahE,RockensteinE,AdameA,AlfordM,CrewsL,HashimotoM,SuebertP,LeeM,GoldsteinJ,ChilcoteT,GamesD,SchenkD(2005)Effectsofalpha-synucleinimmunizationinamousemodelofParkinson’sdisease.NeuronJun16;46(6):857-868.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。