Rabbitpolyclonalantibody
PooledSerumTheantigenisa phosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser239ofhumanATRIP.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
ATRIP,ATRinteractingprotein,bindstoATRtoregulateATRexpression,andisanessentialcomponentoftheDNAdamagecheckpointpathway(Cortezetal,2001).ATRisrecruitedtoDNAlesionsinpartthroughitsassociationwithATRIP,whichinturninteractswiththesingle-strandedDNAbindingproteinRPA(Balletal,2007).DNAreplicationforksmaystallasaresultofDNAdamagecausingphosphorylationofseveralproteins,includingBRCA1whencolocalizingwithATR/ATRIPcomplexandRPA(Venereetal,2007).TheDNAreplicationforkstallcoincideswithBRCA1directlyphosphorylatingATRIPatSer239(Venereetal,2007).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
CortezD,GuntukuS,QinJ,ElledgeSJ(2001)ATRandATRIP:partnersincheckpointsignaling.Science294(5547):1713-6.
BallHL,EhrhardtMR,MordesDA,GlickGG,ChazinWJ,CortexD.(2007)FunctionofaconservedcheckpointrecruitmentdomaininATRIPproteins.MolCelBiol.27(9):3367-77.
VenereM,SynderA,Zgheib,andHalazonetisT.(2007)PhosphorylationofATR-intereactingProtienonSer239MediatesanInteractionwithBreast-OvarianCancerSusceptibility1andCheckpointFunction.CancerRes67(13):6100-5
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。