Rabbitpolyclonalantibody
PooledSerumTheantigenisasyntheticphospho-peptidesurroundingthephosphoSer477ofhumanChe-1.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Che-1,alsoknownasAATF(apoptosis-antagonizingtranscriptionfactor),isaRNApolymeraseII-bindingproteininvolvedinregulatingthetranscriptionfactorE2Fandpromotingcellcycleprogression(Burgdorfetal.,2004).IthasbeensuggestedthatChe-1mayactasaneuroprotectivefactoragainstAbeta-inducedapoptosisbysuppressingtheproductionofreactiveoxidativespecies(Xieetal.,2004).ThecheckpointkinaseChk2hasbeenshowntophosphorylateChe-1atSer477contributingtothemaintenanceoftheG2/McheckpointinducedbyDNAdamage(Brunoetal.,2006).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
BurgdorfS,LeisterP,ScheidtmannKH.(2004)TSG101interactswithapoptosis-antagonizingtranscriptionfactorandenhancesandrogenreceptor-mediatedtranscriptionbypromotingitsmonoubiquitination.JBiolChem.279(17):17524-34
XieJ,GuoQ(2004)AATFprotectsneuralcellsagainstoxidativedamageinducedbyamyloidbeta-peptide.NeurobiolDis.16(1):150-7
BrunoT,DeNicolaF,IezziS,LecisD,D’AngeloC,DiPadovaM,CorbiN,DimizianiL,ZanniniL,JekimovsC,ScarsellaM,PorrelloA,ChersiA,CrescenziM,LeonettiC,KhannaKK,SodduS,FloridiA,PassanantiC,DeliaD,FanciulliM.(2006)Che-1phosphorylationbyATM/ATRandChk2kinasesactivatesp53transcriptionandtheG2/Mcheckpoint.CancerCell.10(6):473-86
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。