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主营:神经科学领域的蛋白及抗体
℡ 4000-520-616
℡ 4000-520-616
PhosphoSolutions/Che-1 (AATF) (Ser477) Antibody/p142-477/100 µl
产品编号:p142-477
市  场 价:¥4574.70
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$345.00
品      牌: PhosphoSolutions
公      司:PhosphoSolutions
公司分类:
PhosphoSolutions/Che-1 (AATF) (Ser477) Antibody/p142-477/100 µl
商品介绍

Rabbitpolyclonalantibody

PooledSerum
Size:
100µl
Formulation:
AffinityPurifiedfromPooledSerum
Specificity:
Rat,Mouse,Human,Xenopus,Bovine,Non-humanprimate,Sheep,Canine
Applications:
WB1:1,000
Species:
Rabbit
MolecularReference:
~66kDa
CiteThisAntibody:
PhosphoSolutionsCat#p142-477,RRID:AB_2492057
Antigen/Purification:ExpandCollapse

Theantigenisasyntheticphospho-peptidesurroundingthephosphoSer477ofhumanChe-1.

Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.

BIOLOGicalSignificance:ExpandCollapse

Che-1,alsoknownasAATF(apoptosis-antagonizingtranscriptionfactor),isaRNApolymeraseII-bindingproteininvolvedinregulatingthetranscriptionfactorE2Fandpromotingcellcycleprogression(Burgdorfetal.,2004).IthasbeensuggestedthatChe-1mayactasaneuroprotectivefactoragainstAbeta-inducedapoptosisbysuppressingtheproductionofreactiveoxidativespecies(Xieetal.,2004).ThecheckpointkinaseChk2hasbeenshowntophosphorylateChe-1atSer477contributingtothemaintenanceoftheG2/McheckpointinducedbyDNAdamage(Brunoetal.,2006).

Storage

100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

GeneralReferences

BurgdorfS,LeisterP,ScheidtmannKH.(2004)TSG101interactswithapoptosis-antagonizingtranscriptionfactorandenhancesandrogenreceptor-mediatedtranscriptionbypromotingitsmonoubiquitination.JBiolChem.279(17):17524-34

XieJ,GuoQ(2004)AATFprotectsneuralcellsagainstoxidativedamageinducedbyamyloidbeta-peptide.NeurobiolDis.16(1):150-7

BrunoT,DeNicolaF,IezziS,LecisD,D’AngeloC,DiPadovaM,CorbiN,DimizianiL,ZanniniL,JekimovsC,ScarsellaM,PorrelloA,ChersiA,CrescenziM,LeonettiC,KhannaKK,SodduS,FloridiA,PassanantiC,DeliaD,FanciulliM.(2006)Che-1phosphorylationbyATM/ATRandChk2kinasesactivatesp53transcriptionandtheG2/Mcheckpoint.CancerCell.10(6):473-86

品牌介绍

PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。

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