RabbitPolyclonalAntibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Human,Non-humanprimate
- Applications:
- WB1:1000
- Species:
- Rabbit
- MolecularReference:
- ~100kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1012-327,RRID:AB_2651148
Antigen/Purification:Collapse
Phosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser327ofhumanCtIP.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephosphopeptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
CtIP,C-terminalbindingprotein-interactingprotein,isaDNAendonucleaseactivatedbydoublestrandedbreaks(DSBs). DSBrepairscanbeperformedbyeitheroneoftwomechanisms;non-homologousendjoining(NHEJ)orhomologousrecombination(HR). NHEJisthepredominantDSBrepairpathwaythroughouttheentirecellcycle,mostimportantlyintheG1phase(Rothkammetal,2003);whileHRisimportantforrepairingDSBsinSandG2phases(Beucheretal,2009).CtIPcontrolsDSBresection;aneventthatonlyoccursinHRduringG2-phase.PhosphorylationofThr847dictatestheresectionefficiency(Huertasetal,2008).FurThermore,ithasbeenfoundthatDSBsundergoresectionandrepairinG1-phasecellsviaaprocessrequiringPlk3phosphorylationofCtIPatSer327andThr847(Bartonetal,2014). SeveraladditionalphosphorylationsiteswithinCtIPhavebeenidentified,buttheirsignificanceintherepairofDNAhaveyettobedetermined.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
OliviaBarton,SteffenCNaumann,RonjaDiemer-Biehs,JuliaKünzel,MonikaSteinlage,SandroConrad,NodarMakharashili,JiadongWang,LinFent,BernardS.Lopez,TanyaT.Paull,JunjieChen,PennyAJeggo,andMarkusLöbrich(2014)Polo-likeKinase3regulatesCtIPduringDNAdouble-strandbreakrepairinG1.JCB, Nov7;206(7):877-894.PMID:25267294
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik，他们在 Paul Greengard 博士的实验室共同工作，Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区，是一支敬业、友好、勤奋的科学家团队，致力于为您提供最优质的抗体和业内客户支持。
本网站将在规定时间内给予删除等相关处理。
