Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Affinitypurified
- Specificity:
- Rat,Mouse,Bovine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- GABRA1
- MolecularReference:
- ~51kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#812-GA1N,RRID:AB_2492100
Antigen/Purification:Collapse
TheantigenisapeptidefromtheN-terminalregionoftheα1-subunitofratGABAAreceptor,conjugatedtokeyholelimpethemocyanin(KLH).
Theantibodyispreparedfromrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Gamma-aminobutyricacid(GABA)istheprimaryinhibitoryneurotransmitterinthecentralnervoussystem,causingahyperpolarizationofthemembranethroughtheopeningofaCl−channelassociatedwiththeGABAAreceptor(GABAA-R)subtype.GABAA-Rsareimportanttherapeutictargetsforarangeofsedative,anxiolytic,andhypnoticagentsandareimplicatedinseveraldiseasesincludingepilepsy,anxiety,depression,andsubstanceabuse.TheGABAA-Risamultimericsubunitcomplex.Todatesixαs,fourβsandfourγs,plusalternativesplicingvariantsofsomeofthesesubunits,havebeenidentified(OlsenandTobin,1990;Whitingetal.,1999;Ogrisetal.,2004).InjectioninoocytesormammaliancelllinesofcRNAcodingforα-andβ-subunitsresultsintheexpressionoffunctionalGABAA-RssensitivetoGABA.However,coexpressionofaγ-subunitisrequiredforbenzo-diazepinemodulation.Thevariouseffectsofthebenzodiazepinesinbrainmayalsobemediatedviadifferentα-subunitsofthereceptor(McKernanetal.,2000;MehtaandTicku,1998;Ogrisetal.,2004;Pöltletal.,2003).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Engin,E.,Zarnowska,E.D.,Benke,D.,Tsvetkov,E.,Sigal,M.,Keist,R.,Bolshakov,V.Y.,Pearce,R.A.,&Rudolph,U.(2015).TonicInhibitoryControlofDentateGyrusGranuleCellsbyα5-ContainingGABAAReceptorsReducesMemoryInterference.TheJournalofNeuroscience,35(40),13698-13712.PMID:26446222
Wyatt,L.R.,Finn,D.A.,Khoja,S.,Yardley,M.M.,Asatryan,L.,Alkana,R.L.,&Davies,D.L.(2014).ContributionofP2X4receptorstoethanolintakeinmaleC57BL/6mice.NeuRochemicalresearch,39(6),1127-1139.PMID:24671605
Herman,M.A.,&Roberto,M.(2014).Cell‐type‐specifictonicGABAsignalingintheratcentralamygdalaisselectivelyalteredbyacuteandchronicethanol.Addictionbiology.Aug.29.PMID:25170988
MridulaRewal,RachelJurd,T.MichaelGill,Dao-YaoHe,DoritRon,andPatriciaH.Janak(2009)alpha-4-ContainingGABAAReceptorsintheNucleusAccumbensMediateModerateIntakeofAlcoholJ.Neurosci.,29:543-549.PMID:19144854
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik，他们在 Paul Greengard 博士的实验室共同工作，Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区，是一支敬业、友好、勤奋的科学家团队，致力于为您提供最优质的抗体和业内客户支持。
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