Mousemonoclonalantibody
TheantigenisrecombinantfulllengthhumanEWSexpressedinandpurifiedfromE.Coli.
TheantibodyisProteinGpurifiedfromculturesupernatant.
TheEwingSarcomaBreakpointRegion1(EWSR1)geneencodestheEWSprotein,whichalongwithFUSandTAF15comprisestheFETfamilyofRNA-bindingproteins. EWSisprimarilyexpressedinthenucleus,buthasalsobeenfoundinthecytoplasmofsecretorycelltypes(Anderssonetal.,2008). DefectsofEWSR1areknowntocauseEwingsarcomaandhavebeenimplicatedinamyotrophiclateralsclerosis(ALS)(Svetonietal.,2016). Recently,EWShasbeenshowntodirectlyinteractwithoncogenicETStranscriptionfactorsinthepromotionofprostatecancer(Kedageetal.,2016).
100µlinPBS+5mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
AnderssonMK,StåhlbergA,ArvidssonY,OlofssonA,SembH,StenmanG,NilssonO,AmanP(2008).ThemultifunctionalFUS,EWSandTAF15proto-oncoproteinsshowcelltype-specificexpressionpatternsandinvolvementincellspreADIngandstressresponse.BMCCellBiol.11:9:37.
SvetoniF,FrisoneP,ParonettoMP(2016)RoleofFETproteinsinneurodegenerativedisorders.RNABiol.2016Nov;13(11):1089-1102
KedageV,SelvarajN,NicholasTR,BudkaJA,PlotnikJP,JerdeTJ,HollenhorstPC(2016)AnInteractionwithEwing’sSarcomaBreakpointProteinEWSDefinesaSpecificOncogenicMechanismofETSFactorsRearrangedinProstateCancer.CellRep.2016Oct25;17(5):1289-1301.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。