Rabbitpolyclonalantibody
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser80ofMECP2(Methyl-CpGBindingProtein2)protein.
Theantibodyisserum.
MECP2(Methyl-CpGBindingProtein2)isachromosomalproteinthatbindstomethylatedDNA.Itcanbindspecificallytoasinglemethyl-CpGpairandisnotinfluencedbysequencesflankingthemethyl-CpGs.MECP2hasbeenshowntomediatetranscriptionalrepressionthroughinteractionwithhistonedeacetylaseandthecorepressorSIN3A.DefectsinMECP2arethecauseofRettsyndrome(RTT).RTTisanX-linkeddominantdisease,itisaprogressiveneurologicdevelopmentaldisorderandoneofthemostcommoncausesofmentalretardationinfemales.RecentstudieshavereportedanewphosphorylationsiteatSer80.PhosphorylationanddephosphorylationofthissitemaybeinvolvedinmodulatingthedynamicfunctionofMECP2inneuronstransitingbetweenrestingandactivestateswithinneuralcircuitsthatunderliebehaviors.(Taoetal.,2009)
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1yearafterdateofreceipt.Avoidfreeze/thawcycles.
Maphis,N.M.,Jiang,S.,Binder,J.,Wright,C.,Gopalan,B.,Lamb,B.T.andBhaskar,K.,2017.WholeGenomeExpressionAnalysisinaMouseModelofTauopathyIdentifiesMECP2asaPossIBLeRegulatorofTauPathology.FrontiersinMolecularNeuroscience,10:69. PMID:28367114
TaoJ,HuK,ChangQ,WuH,ShermanNE,MartinowichK,KloseRJ,SchanenC,JaenischR,WangW,SunYE(2009)PhosphorylationofMeCP2atSerine80regulatesitschromatinassociationandneurologicalfunction.ProcNatlAcadSciUSA.106(12):4882-7. PMID:19225110
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。