Rabbitpolyclonalantibody
PooledSerumTheantigenisa phosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser41ofGap-43.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Gap-43isthoughttohaveanimportantroleindevelopmentandplasticitybecauseitisexpressedathighlevelsinneuronalgrowthconesduringdevelopmentandduringaxonalregeneration(BenowitzandRouttenberg,1997).ThereisalsoevidencefromknockoutanimalsthatGap-43servestoamplifypathfindingsignalsfromthegrowthcone(Strittmatteretal.,1995).Gap-43isthoughttomediateatleastsomeoftheseeffectsviainteractionwithactin.Importantly,phosphorylationatSer41byproteinkinaseC(CatalogNo.1609-PKC)modulatestheinteractionofGap-43withactin(Heetal.,1997)andmayalsoaffectneurotransmitterreleaseduringformsofplasticitylikeLTP(Huloetal.,2002).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
RayuduGopalakrishna,UshaGundimeda,JasonEricSchiffman,andThomasH.McNeill(2008)ADirectRedoxRegulationofProteinKinaseCIsoenzymesMediatesOxidant-inducedNeuritogenesisinPC12CellsJ.Biol.Chem.,May2008;283:14430–14444.PMID:18375950
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。