Chickenpolyclonalantibody
TheantigenisasyntheticpeptidecorrespondingtoaminoacidresiduesfromtheC-terminalregionoftheratGAP43sequence.
TheantibodyistotalIgYfraction.
GAP-43isthoughttohaveanimportantroleindevelopmentandplasticitybecauseitisexpressedathighlevelsinneuronalgrowthconesduringdevelopmentandduringaxonalregeneration(BenowitzandRouttenberg,1997).ThereisalsoevidencefromknockoutanimalsthatGAP-43servestoamplifypathfindingsignalsfromthegrowthcone(Strittmatteretal.,1995).GAP-43isthoughttomediateatleastsomeoftheseeffectsviainteractionwithactin.Importantly,phosphorylationatSer41byproteinkinaseCmodulatestheinteractionofGAP-43withactin(Heetal.,1997)andmayalsoaffectneurotransmitterreleaseduringformsofplasticitylikeLTP(Huloetal.,2002).
TotalIgYfractioninPBS+10mMNaN3.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
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He,Q,Dent,EW,Meiri,KF(1997)ModulationofactinfilamentbehaviorbyGap-43(neuromodulin)isdependentonthephosphorylationstatusofserine41,theproteinkinaseCsite.JNeurosci17:3515-3524.
HuloS,Alberi,S,LauxT,MullerD,CaroniP(2002)ApointmutantofGap-43inducesenhancedshort-termandlongtermhippocampalplasticity.EurJNeurosci15:1976-1982.
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