Rabbitpolyclonalantibody
PooledSerum- Size:
- 200µg
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human
- Applications:
- WB1:1,000IHC1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~48kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#700-GLY,RRID:AB_2492133
Antigen/Purification:Collapse
TheantigenisapeptidefromtheN-terminusregionoftheα1-subunitofratglycinereceptor.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Glycineisanimportantinhibitorytransmitterinthebrainstemandspinalcord.Glycinereceptorsaremembersoftheligand-gatedionchannelfamily(LGICs)thatmediaterapidchemicalneurotransmission(Schofieldetal.,2003).Thebindingofglycinetoitsreceptorproducesalargeincreaseinchlorideconductance,whichcausesmembranehyperpolarization.Glycinereceptorsareanchoredatinhibitorychemicalsynapsesbyacytoplasmicprotein,gephyrin(Fischeretal.,2000).TheglycinereceptorhasbeenusedtogreatadvantageintheidentificationofthebindingsitesforalcoholontheLGICfamilyofproteins(Becksteadetal.,2001;Mihicetal.,1997).Thesereceptorshavealsobeenextremelyusefulinstudiesofsynapticclusteringofreceptors(CraigandLichtman,2001).TheglycinereceptormayalsoactinconcertwithanNMDARsubunittoformanexcitatoryreceptor(Chattertonetal.,2002).
Storage
200µgantibodyvial;lyophilizedfrom5mMammoniumbicarbonate.Theantibodyshouldbereconstitutedin50µlphosphatebufferedsaline(PBS:137mMNaCl,7.5mMNa2HPO4,2.7mMKCl,1.5mMKH2PO4,pH7.4)beforeuse.Afterreconstitutiontheantibodyshouldbealiquotedandstoredat-20°C.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
WickMJ,BleckV,WhatleyVJ,BrozowskiSJ,NixonK,CardosoRA,andValenzuelaCF(1999)Stableexpressionofhumanglycinealpha1andalpha2homomericreceptorsinmouseL(tk-)cells.JNeurosciMethods87(1):97-103.PMID:10065988
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik，他们在 Paul Greengard 博士的实验室共同工作，Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区，是一支敬业、友好、勤奋的科学家团队，致力于为您提供最优质的抗体和业内客户支持。
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