Rabbitpolyclonalantibody
PooledSerumTheantigenisapeptidefromthesubunitregionofthevoltagegatedpotassiumchannel,Kv2.2ofratandXenopus,conjugatedtokeyholelimpethemocyanin(KLH).
TheantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingaSulfo-Link®columnmatrixtowhichthepeptideimmunogenwascoupled.
Voltage-gatedK+channelsareimportantdeterminantsofneuronalmembraneexcitABIlity(Pongs,1999).Moreover,differencesinK+channelexpressionpatternsanddensitiescontributetothevariationsinactionpotentialwaveformsandrepetitivefiringpatternsevidentindifferentneuronalcelltypes.Thedelayedrectifier-type(IK)channels(Kv1.5,Kv2.1,andKv2.2)areexpressedonallneuronalsomataandproximaldendritesandarealsofoundinawidevarietyofnon-neuronalcellstypesincludingpancreaticislets,alveolarcellsandcardiacmyocytes(Hwangetal.,1993;Yanetal.,2004;Michaelevskietal.,2003).Kv2.1andKv2.2formdistinctpopulationsofK+channelsandthesesubunitsarethoughttobeprimarilyresponsIBLeforIKinsuperiorcervicalganglioncells(BlaineandRibera,1998;BurgerandRibera,1996).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Gravagna,N.G.,Knoeckel,C.S.,Taylor,A.D.,Hultgren,B.A.andRibera,A.B.,2008.LocalizationofKv2.2proteininXenopuslaevisembryosandtadpoles.JournalofComparativeNeurology,510(5),pp.508-524.PMID:18680201
D.Guan,T.Tkatch,D.J.Surmeier,W.E.Armstrong,andR.C.Foehring(2007)Kv2subunitsunderlieslowlyinactivatingpotassiumcurrentinratneocorticalpyramidalneurons
J.Physiol.,Jun2007;581:941–960.PMID:17379638
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。