Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser358ofhumaneEF1A2.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
EukaryoticElongationFactoreEF1Aexistsintwovariantforms,eEF1A1andeEF1A2.WhileeEF1A1isalmostubiquitouslyexpressedinhumans,eEF1A2ispredominantlyfoundinheart,brain,andskeletalmuscle(Knudsenetal.,1993).ExpressionofeEF1A2mayhavearoleinovariancancer,asitsexpressionisdrasticallyincreasedinhumanovariantumors(Anandetal.,2002).Duetodifferencesinstructuralmodelsbetweenthetwoisoforms,eEF1A1andeEF1A2likelyhavevariant-specificphosphorylationsites(Soaresetal.,2009).Ribosome-associatedJNKphosphorylatesSer358oneEF1A2topromotedegradationofnewlysynthesizedpolypeptidesbytheproteasome(Gandinetal.,2013).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Gandin,V.,Gutierrez,G.J.,Brill,L.M.,Varsano,T.,Feng,Y.,Aza-Blanc,P.,Au,Q.,McLaughlan,S.,Ferreira,T.A.,Alain,T.,Sonenberg,N., TopisirovicI,&RonaiZA(2013).Degradationofnewlysynthesizedpolypeptidesbyribosome-associatedRACK1/c-JunN-terminalkinase/eukaryoticelongationfactor1A2complex.Molecularandcellularbiology,33(13),2510-2526.PMID:23608534
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。