Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser383ofhumanElk-1.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Elk-1isatranscriptionfactorinvolvedinmediatinggenetranscriptioninresponsetogrowthfactors(HillandTreisman,1995).Elk-1isthoughttobephosphorylatedbyMAPkinaseatSer383andphosphorylationatthissiteisessentialforthetranscriptionalactivityofElk-1(Lietal.,2003).PhosphorylationofElk-1hasalsobeenimplicatedinsynapticplasticityintheadulthippocampus(Thielsetal.,2002).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
HillCS,TreismanR(1995)Transcriptionalregulationbyextracellularsignals:mechanismsandspecificity.Cell80:199-211.
LiQJ,YangSH,MaedaY,SladekFM,SharrocksAD.Martins-GreenM(2003)MAPkinasephosphorylationdependentactivationofElk-1leadstoactivationoftheco-activatorp300.EMBOJ22:281-291.
ThielsE,KanterewiczBI,NormanED,Trzaskos,JM,KlannE(2002)Long-termdepressionintheadulthippocampusinvivoinvolvesactivationofextracellularsignal-regulatedkinaseandphosphorylationofElk-1.JNeurosci22:2054-2062.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。