RabbitPolyclonalAntibody
PooledSerumTheantigenisapeptidefromtheN-terminalregionofthehumaneIF4A2protein.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
TheeIF4A,eukaryoticinitiationfactor4A,areasetofproteins,eIF4A1,eIF4A2,andeIF4A3,thatbelongtotheextensiveDEAD-boxRNAhelicasefamily(Weinsteinetal.,1997).TheyareinvolvedinmanyaspectsofRNAmetabolismbyvirtueoftheirRNA-bindingcapacityandATPaseactivity(Luetal.,2014). eIF4A2isahighlyconservedgeneforoneoftheprotein-synthesisinitiationfactorsinvolvedinthebindingofmRNAtotheribosome(Gingrasetal.,1999).InhumanseIF4A2isexpressedinallnormaltissues,thoughhighlyexpressedinskeletalmuscle(Sudoetal.,1995). StudiessuggesteIF4A2mayplayaroleinsomecancers,specificallybreastandlungcancer,alongwithmelanoma(Shaoyanetal.,2013).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ShaoyanX,JuanjuanY,YalanT,PingH,JianzhongL,QinianW(2013)DownregulationofeIF4A2inNon-Small-CellLungCancerAssociateswithPoorPrognosis.ClinLungCancer,14(6):658-65.
GingrasAC,RaughtB,SonenbergN(1999)eIF4initiationfactors:EffectorsofmRNARecruitmenttoRibosomesandRegulatorsofTranslation.AnnuRevBiochem,68:913-63.
WeinsteinDC,HonoreE,Hemmati-BrivanlouA(1997)EpidermalInductionandInhibitionofNeuralFatebyTranslationInitiationFactor4AIII.Development124:4235-4242.
SudoK,TakahashiE,NakamuraY(1995)IsolationandMappingoftheHumaneIF4A2GeneHomologoustotheMurineProteinSynthesisInitiationFactor4A-IIgeneEif4a2.CytogenetCellGenet,71:385-8.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。