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主营:神经科学领域的蛋白及抗体
℡ 4000-520-616
℡ 4000-520-616
PhosphoSolutions/Eukaryotic Initiation Factor 4A2 (eIF4A2)/495-eIF4A2/100 µl
产品编号:495-eIF4A2
市  场 价:¥4574.70
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$345.00
品      牌: PhosphoSolutions
公      司:PhosphoSolutions
公司分类:
PhosphoSolutions/Eukaryotic Initiation Factor 4A2 (eIF4A2)/495-eIF4A2/100 µl
商品介绍

RabbitPolyclonalAntibody

PooledSerum
Size:
100µl
Formulation:
AffinityPurifiedfromPooledSerum
Specificity:
Bovine,Human,Mouse,Non-humanprimate,Rat
Applications:
WB1:1000
Species:
Rabbit
MolecularReference:
~46kDa
CiteThisAntibody:
PhosphoSolutionsCat#495-eIF4A2,RRID:AB_2630324
Antigen/Purification:ExpandCollapse

TheantigenisapeptidefromtheN-terminalregionofthehumaneIF4A2protein.

Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.

 

BIOLOGicalSignificance:ExpandCollapse

TheeIF4A,eukaryoticinitiationfactor4A,areasetofproteins,eIF4A1,eIF4A2,andeIF4A3,thatbelongtotheextensiveDEAD-boxRNAhelicasefamily(Weinsteinetal.,1997).TheyareinvolvedinmanyaspectsofRNAmetabolismbyvirtueoftheirRNA-bindingcapacityandATPaseactivity(Luetal.,2014). eIF4A2isahighlyconservedgeneforoneoftheprotein-synthesisinitiationfactorsinvolvedinthebindingofmRNAtotheribosome(Gingrasetal.,1999).InhumanseIF4A2isexpressedinallnormaltissues,thoughhighlyexpressedinskeletalmuscle(Sudoetal.,1995). StudiessuggesteIF4A2mayplayaroleinsomecancers,specificallybreastandlungcancer,alongwithmelanoma(Shaoyanetal.,2013).

Storage

100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

GeneralReferences

Lu,W.T.,Wilczynska,A.,Smith,E.,&Bushell,M.(2014).ThediverserolesoftheeIF4Afamily:youarethecompanyyoukeep.BiochemicalSocietyTransactions,42(1),166-172.

ShaoyanX,JuanjuanY,YalanT,PingH,JianzhongL,QinianW(2013)DownregulationofeIF4A2inNon-Small-CellLungCancerAssociateswithPoorPrognosis.ClinLungCancer,14(6):658-65.

GingrasAC,RaughtB,SonenbergN(1999)eIF4initiationfactors:EffectorsofmRNARecruitmenttoRibosomesandRegulatorsofTranslation.AnnuRevBiochem,68:913-63.

WeinsteinDC,HonoreE,Hemmati-BrivanlouA(1997)EpidermalInductionandInhibitionofNeuralFatebyTranslationInitiationFactor4AIII.Development124:4235-4242.

SudoK,TakahashiE,NakamuraY(1995)IsolationandMappingoftheHumaneIF4A2GeneHomologoustotheMurineProteinSynthesisInitiationFactor4A-IIgeneEif4a2.CytogenetCellGenet,71:385-8.

 

品牌介绍

PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。

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