Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser679/683ofthehumanFAM129Bsequence.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
FAM129B,alsoknownasNiban-likeprotein1,belongstoapoorlycharacterizedproteinfamilywithunknowncategoryandfunction.IncreasedexpressionoftheNibangenehasbeenobservedinrenalcarcinomas(Adachietal.,2004;Sunetal.,2007).SuppressionofFAM129BexpressioninHeLacellshasbeenseentopromoteapoptosis,suggestingthatitcanmodulatecelldeathsignaling,andmaybeinvolvedintheERstressresponse(Sunetal.,2007).FAM129Bisalsoup-regulatedinvarioustypesofthyroidtumorsandHashimoto’sthyroiditis(Matsumotoetal.,2006).IthasbeensuggestedthattheMAPkinasedependentphosphorylationofFAM129Bisimportantincontrollingmelanomacells,asinhibitionofB/Raf/MKK/ERKinmelanomacellsrepressesinvasion(Oldetal.,2009).ItisbelievedthatphosphorylatedFAM129Bnotonlyderepressesinvasion,butalsoregulateseventsthatpromoteinvasion(Oldetal.,2009).
Liquidin100mMGlycine,200mMTris,pH8.0.Containsnopreservative.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
AdachiH,MajimaS,KonS,KobayashiT,KajinoK,MitaniH,HirayamaY,ShiinaH,IgawaM,HinoO.Nibangeneiscommonlyexpressedintherenaltumors:anewcandidateMarkerforrenalcarcinogenesis.Oncogene.2004Apr22;23(19):3495-500.
SunGD,KobayashiT,AbeM,TadaN,AdachiH,SiotaA,Totsuka,HinoO.Theendoplasmicreticulumstress-inducIBLeproteinNibanregulateseIF2alphaandS6K1/4E-BP1phosphorylation.BiochemBiophysResCommun.2007Aug17;360(1):181-7.
MatsumotoF,FujiiH,AbeM,KajinoK,KobayashiT,MatsumotoT,IkedaK,HinoO.Anoveltumormarker,Niban,isexpressedinsubsetsofthyroidtumorsandHashimoto’sthyroiditis.HumanPathology.2006Dec;37(12):1592-600.
OldW,ShabbJ,HouelS,WangH,CoutsK,YenC,LitmanE,CroyC,Meyer-ArendtK,MirandaJ,BrownR,WitzeE,SchweppeR,ResingK,AhnN.FunctionalProteomicsIdentifiesTargetofPhosphorylationbyB-RafSignalinginMelanoma.MolecularCell.2009Apr10(34):115-131
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。