Rabbitpolyclonalantibody
PooledSerumTheantigenisasyntheticphospho-peptidesurroundingthephosphoSer94ofhumanAR.
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns
Theandrogenreceptor(AR)isaDNA-bindingtranscriptionfactorthatregulatesgenescriticalforthedevelopmentandmaintenanceofthemalesexualphenotype.Defectsinandrogenreceptorhavebeenshowntoplayaroleinprostatecancer,andinhibitionofARactivitythroughmodulationofsignaltransductionpathwaysmaydelayprostatecancerprogression(HeinleinandChang2004).Multiplephosphorylationsiteshavebeenidentifiedontheandrogenreceptorthataffectcross-talkbetweengrowthfactorsignalingandandrogeninprostatedevelopmentandcancer(Gioelietal.,2002).Oneofthesesites,atSer94,appearsconstitutivelyphosphorylatedandexhibitsnoresponsetotreatmentswithstimulatinghormone(Gioelietal.,2002).ThesiteatSer94isuniqueamongtheARphosphorylationsitesinthatitdoesnotachieveamaximallevelofphosphorylationbetweentranslationandtheinitialroundofnuclearimport,havingastrongbiasforandrogen-independentphosphorylationinthecytoplasm(Kesleretal.,2007).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
HeinleinC,ChangC.(2004)AndrogenReceptorinProstateCancer.EndocrineReviews.25(2):276-308
GioeliD,FicarroS,KwiekJ,AaronsonD,HancockM,CatlingA,WhiteF,ChristianR,SettlageR,ShabanowitzJ,HuntD,WeberM.(2002)AndrogenReceptorPhosphorylation,RegulationandIndentificationofthePhosphorylationSites.JBiolChem.277(32):29304-29314
KeslerC,GioeliD,ConawayM,WeberM,PaschalB.(2007)SubcellularLocalizationModulatesActivationFunction1DomainPhosphorylationintheAndrogenReceptor.MolecularEndocrinology.21(9):2071-2084
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。