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主营:神经科学领域的蛋白及抗体
℡ 4000-520-616
℡ 4000-520-616
PhosphoSolutions/Progesterone Receptor (Ser294) Antibody/p205-294/100 µl
产品编号:p205-294
市  场 价:¥4574.70
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$345.00
品      牌: PhosphoSolutions
公      司:PhosphoSolutions
公司分类:
PhosphoSolutions/Progesterone Receptor (Ser294) Antibody/p205-294/100 µl
商品介绍

Mousemonoclonalantibody

Size:
100µl
Formulation:
ProteinGPurified
Specificity:
Human,Non-humanprimate
Applications:
WB1:1,000IHC1:1,000
Species:
Mouse
MolecularReference:
~90kDaand~120kDa
CiteThisAntibody:
PhosphoSolutionsCat#p205-294,RRID:AB_2492216
Antigen/Purification:ExpandCollapse

TheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephosphoSer294ofhumanprogesteronereceptor.

TheantibodyispreparedbyaffinitypurificationusingaProteinGcolumn.

BIOLOGicalSignificance:ExpandCollapse

Thereisaccumulatingevidencetosuggestthatprogesteroneplaysanessentialroleintheregulationofgrowthanddifferentiationofmammaryglandsandthusmayplayakeyroleinbreastcancer(Edwards,2005).Thebiologicalresponsetoprogesteroneismediatedbytwodistinctformsofthehumanprogesteronereceptor(PR-AandPR-Bforms).Inmostcellcontexts,theBformfunctionsasatranscriptionalactivator,whereastheAformfunctionsasatranscriptionalinhibitorofsteroidhormones(Attiaetal.,2000;Linetal.,2003).RecentlyithasbeendemonstratedthatthereisdifferentialhormonedependentregulationofthephosphorylationoftheAandBformsofthereceptor(Clemmetal.,2000).TreatmentofT47DbreastcancercellswithprogestinagoNISTincreasesthephosphorylationofSer190andSer294withdifferentkinetics.Thesephosphorylationeventsmaydifferentiallyaffectthetranscriptionalactivityofthereceptor.

Storage

100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

GeneralReferences

AttiaGR,ZeitounK,EdwardsD,JohnsA,CarrBR,BulunSE(2000)ProgesteronereceptorisoformAbutnotBisexpressedinendometriosis.JClinEndocrinolMetab85:2897-2902.ClemmDL,ShermanL,BoonyaratanakornkitV,SchraderWT,WeigelNL,EdwardsDP(2000)Differentialhormone-dependentphosphorylationofprogesteronereceptorAandBformsrevealedbyaphosphoserinesite-specificmonoclonalantibody.MolEndocrinol14:52-65.

EdwardsDP(2005)Regulationofsignaltransductionpathwaysbyestrogenandprogesterone.AnnuRevPhysiol67:335-376.LinVC,WoonCT,AwSE,GuoC(2003)Distinctmolecularpathwaysmediateprogesterone-inducedgrowthinhibitionandfocaladhesion.Endocrinology144:5650-5657

品牌介绍

PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。

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