Mousemonoclonalantibody
TheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephosphoSer294ofhumanprogesteronereceptor.
TheantibodyispreparedbyaffinitypurificationusingaProteinGcolumn.
Thereisaccumulatingevidencetosuggestthatprogesteroneplaysanessentialroleintheregulationofgrowthanddifferentiationofmammaryglandsandthusmayplayakeyroleinbreastcancer(Edwards,2005).Thebiologicalresponsetoprogesteroneismediatedbytwodistinctformsofthehumanprogesteronereceptor(PR-AandPR-Bforms).Inmostcellcontexts,theBformfunctionsasatranscriptionalactivator,whereastheAformfunctionsasatranscriptionalinhibitorofsteroidhormones(Attiaetal.,2000;Linetal.,2003).RecentlyithasbeendemonstratedthatthereisdifferentialhormonedependentregulationofthephosphorylationoftheAandBformsofthereceptor(Clemmetal.,2000).TreatmentofT47DbreastcancercellswithprogestinagoNISTincreasesthephosphorylationofSer190andSer294withdifferentkinetics.Thesephosphorylationeventsmaydifferentiallyaffectthetranscriptionalactivityofthereceptor.
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
AttiaGR,ZeitounK,EdwardsD,JohnsA,CarrBR,BulunSE(2000)ProgesteronereceptorisoformAbutnotBisexpressedinendometriosis.JClinEndocrinolMetab85:2897-2902.ClemmDL,ShermanL,BoonyaratanakornkitV,SchraderWT,WeigelNL,EdwardsDP(2000)Differentialhormone-dependentphosphorylationofprogesteronereceptorAandBformsrevealedbyaphosphoserinesite-specificmonoclonalantibody.MolEndocrinol14:52-65.
EdwardsDP(2005)Regulationofsignaltransductionpathwaysbyestrogenandprogesterone.AnnuRevPhysiol67:335-376.LinVC,WoonCT,AwSE,GuoC(2003)Distinctmolecularpathwaysmediateprogesterone-inducedgrowthinhibitionandfocaladhesion.Endocrinology144:5650-5657
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。