Mousemonoclonalantibody
TheantigenisapeptidecorrespondingtoaminoacidresiduesfromtheN-terminalregionofhumanretinoicacidreceptor,α-isotype.
TheantibodyispreparedfrommouseascitesbyammoniumsulfateprecipitationfollowedaffinitypurificationonaProteinGcolumn.
Retinoicacid(RA;activemetaboliteofvitaminA)playsaprominentroleinregulatingthetransitionofproliferatingprecursorcells(suchascarcinomacellsandneuronalprecursors)topostmitoticdifferentiatedcells(Joshietal.,2005).TheretinoidXreceptors(RXRs)family(RXRα,βandγ),preferentiallybind9-cis-RAandregulategenetranscriptionbyformingheterodimerswithasecondfamilyofRAreceptors.RAshavebeensuggestedtopotentiallyplayatherapeuticroleincervicalcancer(Abuetal.,2005).RAsareknowntoplaykeyrolesinneuronaldevelopmentandanincreasingbodyofevidenceindicatesthatretinoidsignalingmayregulatesynapticplasticityandassociatedlearningandmemorybehaviors(LaneandBailey,2005).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
AbuJ,BatuwangalaM,HerbertK,SymondsP(2005)Retinoicacidandretinoidreceptors:potentialchemopreventiveandtherapeuticroleincervicalcancer.LancetOncol6:712-720.
JoshiS,GuleriaR,PanJ,DipetteD,SinghUS(2005)Retinoicacidreceptorsandtissue-trans-glutaminasemediateshort-termeffectofretinoicacidonmigrationandinvasionofneuroblastomaSH-SY5Ycells.Oncogeneadvanceonlinepublication12September2005;doi:10.1038/sj.onc.1209027.
LaneMA,BaileySJ(2005)Roleofretinoidsignallingintheadultbrain.ProgNeurobiol75:275-293.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。