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主营:神经科学领域的蛋白及抗体
℡ 4000-520-616
℡ 4000-520-616
PhosphoSolutions/Troponin I (cardiac) Antibody/2010-TNI/100 µl
产品编号:2010-TNI
市  场 价:¥3911.70
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$295.00
品      牌: PhosphoSolutions
公      司:PhosphoSolutions
公司分类:
PhosphoSolutions/Troponin I (cardiac) Antibody/2010-TNI/100 µl
商品介绍

Rabbitpolyclonalantibody

PooledSerum
Size:
100µl
Formulation:
Serapooled,neatserum
Specificity:
Rat,Mouse
Applications:
WB1:2,000
Species:
Rabbit
MolecularReference:
~25kDa
CiteThisAntibody:
PhosphoSolutionsCat#2010-TNI,RRID:AB_2492267
Antigen/Purification:ExpandCollapse

Theantigenisa  fusionproteinofthemousecardiactroponinIholoprotein.

Theantibodyisunpurifiedneatserum.

BIOLOGicalSignificance:ExpandCollapse

TroponinI(cTnI)is1of3subunits,alongwithtroponinC(TnC)andtroponinT(TnT)oftroponincomplexfoundincardiacmuscle.cTnIbindstoactininthinmyofilamentstoholdthetroponin-tropomyosincomplexinplace.PhosphorylationofcardiacisoformofTnIatserines22,23intheuniqueamino-terminalendmoleculedecreasesthecalciumsensitivityofthesarcomere,promotescalciumdissociationfromtroponinCandbyextensionenhancesratesofcross-bridgecyclinganddiastolicrelaxation(Noland,Jr.etal.,1995;Nolandetal.,1989).Inaddition,studiesusingreconstitutedfibersandmutationalanalysishaveshownthatPKCphosphorylationofTnI(largelyatSer43)inhibitstheactin-crossbridgereactionandreducestheCa++dependentactomyosinATPaserateaswellasthecalciumsensitivityofforcegeneration(Noland,Jr.andKuo,1991).PhosphorylationatThr144(mediatedbyseveralPKCisoforms)reducesmaximaltensiondevelopmentandcross-bridgecyclingrates(Sumandeaetal.,2008).Importantly,changesinthephosphorylationateachofthesesiteshavebeenshowntobestage-specificwithregardtocardiacdiseaseprogression(Walkeretal.,2010).

Storage

100μlneatserum.Adequateamountofmaterialtoconduct10-miniWesternblots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

GeneralReferences

NolandTA,Jr.,GuoXD,RaynorRL,JideamaNM,Averyhart-FullardV,SolaroRJ,KuoJF(1995)CardiactroponinImutants–PhosphorylationbyproteinkinasesCandAandregulationofCa2+-stimulatedMgATPaseofreconstitutedactomyosinS-1.JBiolChem270:25445-25454.

NolandTA,Jr.,KuoJF(1991)ProteinkinaseCphosphorylationofcardiactroponinIortroponinTinhibitsCa2(+)-stimulatedactomyosinMgATPaseactivity.JBiolChem266:4974-4978.

NolandTAJr,RaynorRL,KuoJF(1989)IdentificationofsitesphosphorylatedinbovinecardiactroponinIandtroponinTbyproteinkinaseCandcomparativesubstrateactivityofsyntheticpeptidescontainingthephosphorylationsites.JBiolChem264:20778-20785.

SumandeaMP,RybinVO,HinkenAC,WangC,KobayashiT,HarletonE,SievertG,BalkeCW,FeinmarkSJ,SolaroRJ,SteinbergSF(2008)TyrosinephosphorylationmodifiesproteinkinaseCdelta-dependentphosphorylationofcardiactroponinI.JBiolChem283:22680-22689.

WalkerLA,WalkerJS,AmblerSK,ButtrickPM(2010)Stage-specificchangesinmyofilamentproteinphosphorylationfollowingmyocardialinfarctioninmice.JMolCellCardiol48:1180-1186

品牌介绍

PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。

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