Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser43ofmousetroponinI,cardiac(cTnI).
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
TroponinI(TnI)is1of3subunits,alongwithtroponinC(TnC)andTroponinT(TnT)oftroponincomplexfoundincardiac(cTnI)andfastskeletal(fsTnI)muscle(Nolandetal,1995).ProteinkinaseC-mediatedphosphorylationofcardiacmyofilamentproteinshasbeenshowntodepresstheactomyosininteractionandmaybeimportantduringheartfailure.(Montgomeryetal,2002)StudiesindicatethatphosphorylationofSer43andSer45ofcardiactroponinIplaysasubstantialroleinthePKCmediateddepression.(Montgomeryetal,2002).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Thoemmes,S.F.,Stutzke,C.A.,Du,Y.,Browning,M.D.,Buttrick,P.M.,&Walker,L.A.(2014).Characterizationandvalidationofnewtoolsformeasuringsite-specificcardiactroponinIphosphorylation.Journalofimmunologicalmethods,403(1),66-71.PMID:24291343
Walker,L.A.,Fullerton,D.A.,&Buttrick,P.M.(2013).Contractileproteinphosphorylationpredictshumanheartdiseasephenotypes.AmericanJournalofPhysiology-HeartandCirculatoryPhysiology,304(12),H1644-H1650.PMID:23564307
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。