Mousemonoclonalantibody
TheantigenisafulllengthrecombinanthumandoublecortinexpressedinandpurifiedfromE.coli.
TheantibodyisProteinGpurifiedfromculturesupernatant.
Doublecortin,orDCX,isamicrotubuleassociatedproteinthatisexpressedalmostexclusivelyinveryearlyneuronaldevelopment(Brownetal.,2003),makingitanexcellentMarkerfordevelopingneuronalcells.DefectsintheDCXgeneleadtoX-linkedlissencephalywhichischaracterizedbyalackofnormalfoldsonthesurfaceofthebrainresultinginasmoothcerebralcortexcausedbyabnormalmigrationofneuronsduringdevelopment(desPortesetal.,1998;Gleesonetal.,1998).
100µlinPBS+10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
BrownJP,Couillard-DesprésS,Cooper-KuhnCM,WinklerJ,AignerL,KuhnHG(2003)Transientexpressionofdoublecortinduringadultneurogenesis.JCompNeurol.Dec1;467(1):1-10.
desPortesV,PinardJM,BilluartP,VinetMC,KoulakoffA,CarriéA,GelotA,DupuisE,MotteJ,Berwald-NetterY,CatalaM,KahnA,BeldjordCandChellyJ.(1998)AnovelCNSgenerequiredforneuronalmigrationandinvolvedinX-linkedsubcorticallaminarheterotopiaandlissencephalysyndrome.Cell92:51-61.
GleesonJG,AllenKM,FoxJW,LampertiED,BerkovicS,SchefferI,CooperEC,DobynsWB,MinnerathSR,RossMEandWalshCA.(1998)Doublecortin,abrain-specificgenemutatedinhumanX-linkedlissencephalyanddoublecortexsyndrome,encodesaputativesignalingprotein.Cell92:63-72.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。