Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Affinitypurified
- Specificity:
- Rat,Human
- Applications:
- WB1:1,000IHC1:250
- Species:
- Rabbit
- MolecularReference:
- ~43kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#2000-TDP,RRID:AB_2492258
Antigen/Purification:Collapse
TheantigenisasyntheticpeptideC-terminaltothecaspase-cleavagesite(betweenD219andV220)ofhumanTDP-43.
Theantibodyisaffinitypurified.
BIOLOGicalSignificance:Collapse
TDP43(TarDNABinding43,TARDBP)wasoriginallyidentifiedasaproteinwhichbindstothe“transactivationresponse”(TAR)sequencefoundinthelongterminalrepeatoftheHIV-1virusgenome(Ouetal.,1995).UVcross-linkingofHeLacellextractrevealeda43kDaproteinwhichwasclonedandsequencedandshowntocontaintwocopiesofthe~90aminoacidRRMdomain.RRMisanacronymforRNARecognitionMotif,andthisdomainisfoundinmanyproteinswhichbindsinglestrandedRNAandsomewhichbindsinglestrandedDNA.Northernblotsshowedthattheproteinisubiquitousintissueexpression.MuchinteresthasbeenfocusedonTDP43recentlyduetoitsassociationwiththeinclusionsseeninfrontotemporallobardegenerationandAmyotrophicLateralSclerosis(Neumannetal.,2006).Theproteinispresentintheseinclusionsinpartiallydegraded,hyperphosphorylatedandubiquitinatedforms.
Storage
LiquidinPhosphateBuffercontaining30%glycerol.
Forlongtermstorage–80°Cisrecommendedinundilutedaliquots.Stableforuptosixmonthsafterdateofreceipt.Avoidfreeze/thawcycles.
ProductSpecificReferences
FormanMS,TrojanowskiJQandLeeVM-Y.(2007).TDP-43:anovelneurodegenerativeproteinopathy.CurrentOpinionsinNeurobiology17:548-55.PMID:17936612
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik，他们在 Paul Greengard 博士的实验室共同工作，Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区，是一支敬业、友好、勤奋的科学家团队，致力于为您提供最优质的抗体和业内客户支持。
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