Chickenpolyclonalantibody
Theantigenis a recombinantfulllengthhumanUCHL1purifiedfromE.coli.
Theantibodyis TotalIgYfraction.
UbiquitinC-terminalhydrolase1(UCHL1)isalsoknownasubiquitincarboxylesteraseL1,ubiquitinthiolesterase,neuron-specificproteinPGP9.5andPark5.Itwasoriginallyidentifiedasamajorcomponentoftheneuronalcytoplasmfrom2-dimensionalgelanalysisofbraintissues,andwasgiventhenamePGP9.5(1).ItwaslaterfoundthatubiquitinC-terminalhydrolaseenzymeactivitywasassociatedwiththePGP9.5protein(2).TheubiquitinC-terminalhydrolasescleaveubiquitinfromothermolecules.Regulationoftheubiquitinpathwayisveryimportantandmanydiseasestatesareassociatedwithdefectsinthispathway.GeneticknockoutofUCHL1inmiceresultsinamotorneurondegenerationsimilartothespontaneousgracileaxonaldystrophy(gad)mutantmice(3).PointmutationsintheUCHL1geneareassociatedwithsomeformsofhumanParkinson’sdisease(4).SinceUCHL1isheavilyexpressedinneurons,itisreleasedinlargeamountsfollowinginjuryordegeneration,sothedetectionofUCHL1inCSFandotherbodilyfluidscanbeusedasabioMarker.
Liquid.Contains10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
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WilkinsonKD,LeeKM,DeshpandeS,Duerksen-HughesP,BossJM,PohlJ.TheneuronspecificproteinPGP9.5isaubiquitincarboxyl-terminalhydrolase.Science.1989246:670-3(1989).
KuriharaLJ,KikuchiT,WadaK,TilghmanSM.LossofUch-L1andUch-L3leadstoneurodegeneration,posteriorparalysisanddysphagia.HumMolGenet.10:1963-70(2001).
LiuY,FallonL,LashuelHA,LiuZ,LansburyPTJr.TheUCH-L1geneencodestwoopposingenzymaticactivitiesthataffectalpha-synucleindegradationandParkinson’sdiseasesusceptibility.Cell111:209-18(2002)
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。