Mousemonoclonalantibody
TheantigenisarecombinanthumanvimentinpurifiedfromE.coli.
TheantibodyisProteinGpurified.
Vimentinisthemajorproteinsubunitofthe10nmorintermediatefilaments(IFs)foundinmanykindsofmesenchymalandepithelialcellsaswellasdevelopingneuronalandastrocyticprecursorcellsintheCNS.Vimentinisthoughttobecriticallyinvolvedinlymphocyteadhesionandtransmigration(NieminenMetal.2006).CopolymersarefrequentlyformedbetweenvimentinandotherIFs,suchasGFAP(inmanykindsofastrocytes),desmin(inmusclecells)andneurofilamentproteins(indevelopingneurons).AntibodiestovimentinareusefulinstudiesofstemcellsandgenerallytorevealthefilamentousCytoskeleton.Recentstudiessuggestthatvimentinaffectsprostatecancercellsmotilityandinvasiveness(Zhaoetal.2008).
Liquid.Contains10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
NieminenM,HenttinenT,MerinenM,Marttila-IchiharaF,ErikssonJE,JalkanenS(2006)Vimentinfunctioninlymphocyteadhesionandtranscellularmigration.NatCellBiol8(2):156-62.
ZhaoY,YanQ,LongX,ChenX,WangY(2008)Vimentinaffectsthemobilityandinvasivenessofprostatecancercells.CellBiochemFunct.May8[Epubaheadofprint]
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