Rabbitpolyclonalantibody
Theantigenisa Fusionproteinfromthecytoplasmicloopoftheγ2-subunitofratGABAA receptor.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationusingacolumntowhichthefusionproteinimmunogenwascoupled.
Gamma-aminobutyricacid(GABA)istheprimaryinhibitoryneurotransmitterinthecentralnervoussystem,causingahyperpolarizationofthemembranethroughtheopeningofaCl−channelassociatedwiththeGABAA receptor(GABAA-R)subtype.GABAA-Rsareimportanttherapeutictargetsforarangeofsedative,anxiolytic,andhypnoticagentsandareimplicatedinseveraldiseasesincludingepilepsy,anxiety,depression,andsubstanceabuse.TheGABAA-Risamultimericsubunitcomplex.Todatesixαs,fourβsandfourγs,plusalternativesplicingvariantsofsomeofthesesubunits,havebeenidentified(OlsenandTobin,1990;Whitingetal.,1999;Ogrisetal.,2004).InjectioninoocytesormammaliancelllinesofcRNAcodingforα-andβ-subunitsresultsintheexpressionoffunctionalGABAA-RssensitivetoGABA.However,coexpressionofaγ-subunitisrequiredforbenzodiazepinemodulation.Thevariouseffectsofthebenzodiazepinesinbrainmayalsobemediatedviadifferentα-subunitsofthereceptor(McKernanetal.,2000;MehtaandTicku,1998;Ogrisetal.,2004;Pöltletal.,2003).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
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Mao,Y.T.,&Pallas,S.L.(2013).Cross-modalplasticityresultsinincreasedinhibitioninprimaryauditorycorticalareas.Neuralplasticity,2013.PMID:24288625
Wen-HaiChou,DanWang,ThomasMcMahon,Zhan-HengQi,MaengseokSong,ChaoZhang,KevanM.Shokat,andRobertO.Messing(2010)GABAAReceptorTraffickingIsRegulatedbyProteinKinaseCandtheN-Ethylmaleimide-SensitiveFactorJ.Neurosci.,30:13955–13965.PMID:20962217
PöltlA,HauerB,FuchsK,TretterV,SieghartW(2003)SubunitcompositionandquantitativeimportanceofGABAAreceptorsubtypesinthecerebellumofmouseandrat.JNeuRochem87:1444-1455.PMID:14713300
WhitingPJ,BonnertTP,McKernanRM,FarrarS,LeBourdellèsB,HeavensRP,SmithDW,HewsonL,RigbyMR,SirinathsinghjiDJS,ThompsonSA,WaffordKA(1999)MolecularandfunctionaldiversityoftheexpandingGABAAreceptorgenefamily.AnnNYAcadSci868:645-653.PMID:10414349
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。