Rabbitpolyclonalantibody
TheantigenisapeptidefromtheC-terminalregionofratmGluR5andratmGluR1a.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
Themetabotropicglutamatereceptors(mGluRs)arekeyreceptorsinthemodulationofexcitatorysynaptictransmissioninthecentralnervoussystem.Theyareimplicatedinmanyformsofneuralplasticityaswellaslearningandmemoryanddrugabuse(Bhattacharyaetal.,2004;Francesconietal.,2004;WilsonandNicoll,2001).GroupImetabotropicglutamatereceptors(consistingofmGluR1andmGluR5)areG-protein-coupledneurotransmitterreceptorsthatarelocalizedintheperisynapticregionofthepostsynapticmembrane.Whenactivated,GroupImGluRsleadtostimulationofphospholipaseandactivationofProteinKinaseC.Incontrast,activationofGroupIImetabotropicreceptors(mGluR2andmGluR3)leadstoinhibitionofadenylatecyclase.ThemGluR1receptormayalsobecriticallyinvolvedinlimitingthedeleteriouseffectsofexcitotoxicity(Blaabjergetal.,2003).Incontrast,themGluR5receptorappearstobeessentialforlatephaseLTPinareaCA1ofthehippocampus(Francesconietal.,2004).
100µlin200mMTris-Glycine(pH7.5),0.1%sodiumazide.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
BhattacharyaM,BabwahAV,GodinC,AnborghPH,DaleLB,PoulterMO,FergusonSSG(2004)RalandphospholipaseD2-dependentpathwayforconstitutivemetabotropicglutamatereceptorendocytosis.JNeurosci24:8752-8761.
BlaabjergM,FangLW,ZimmerJ,BaskysA(2003)NeuroprotectionagainstNMDAexcitotoxicitybygroupImetabotropicglutamatereceptorsisassociatedwithreductionofNMDAstimulatedcurrents.ExpNeurol183:573-580.
FrancesconiW,CammalleriM,SannaPP(2004)Themetabotropicglutamatereceptor5isnecessaryforlate-phaselong-termpotentiationinthehippocampalCA1region.BrainRes1022:12-18.
WilsonRI,NicollRA(2001)EndogenouscannABInoidsmediateretrogradesignallingathippocampalsynapses.Nature(London)410:588-592.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。