Rabbitpolyclonalantibody
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingphospho-Ser862ofratmGluR7.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptidecolumns.
Metabotropicglutamatereceptors(mGluRs)arekeyreceptorsinthemodulationofexcitatorysynaptictransmissioninthecentralnervoussystem.Theyareimplicatedinmanyformsofneuralplasticityaswellaslearningandmemoryanddrugabuse(Bhattacharyaetal.,2004;Francesconietal.,2004;WilsonandNicoll,2001).ThemGluRsaredividedintothreegroupsbasedonsequenceidentityandpharmacologicalproperties:groupI(mGluR1andmGluR5)arelocalizedintheperisynapticregionofthepostsynapticmembrane,whereasgroupII(mGlur2andmGluR3)andgroupIII(mGluR4,6,7and8)arelocalizedpredominantlyatpresynapticterminals.PKCphosphorylationofserine862onmGluR7hasbeenshowntobecriticalforstABIlizingreceptorsurfaceexpressionandpromotingbindingtothesynapticPDZ-domain-containingproteinPICK1(Suhetal.,2008).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
BhattacharyaM,BabwahAV,GodinC,AnborghPH,DaleLB,PoulterMO,FergusonSSG(2004)RalandphospholipaseD2-dependentpathwayforconstitutivemetabotropicglutamatereceptorendocytosis.JNeurosci24:8752-8761.
FrancesconiW,CammalleriM,SannaPP(2004)Themetabotropicglutamatereceptor5isnecessaryforlate-phaselong-termpotentiationinthehippocampalCA1region.BrainRes
WilsonRI,NicollRA(2001)Endogenouscannabinoidsmediateretrogradesignallingathippocampalsynapses.Nature(London)410:588-592.
SuhYH,PelkeyKA,LavezzariG,RochePA,HuganirRL,McBainCJ,RocheKW.(2008)CorequirementofPICK1bindingandPKCphosphorylationforstablesurfaceexpressionofthemetabotropicglutamatereceptormGluR7.Neuron.58(5):736-48
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。