Rabbitpolyclonalantibody
PooledSerum- Size:
- 25µg
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Non-humanprimate,Canine
- Applications:
- WB1:1,000IHC1:1,000to1:2,000
- Species:
- Rabbit
- GeneName:
- GRIN1
- MolecularReference:
- ~120kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1505-C1,RRID:AB_2492166
Antigen/Purification:Collapse
TheantigenisapeptidefromtheNR1subunit,C1splicevariantinsertoftheNMDAreceptor.
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
TheionchannelsactivatedbyglutamatethataresensitivetoN-methyl-Daspartate(NMDA)aredesignatedNMDAReceptors(NMDAR).TheNMDARplaysanessentialroleinmemoryandneuronaldevelopmentandithasalsobeenimplicatedinseveraldisordersofthecentralnervoussystemincludingAlzheimer’s,epilepsyandischemicneuronalcelldeath(Grosshansetal.,2002;Wentholdetal.,2003;CarrollandZukin,2002).ThereareanumberofdifferentsplicevariantsoftheNR1-Subunit(Foldesetal.,1994;ZukinandBennett,1995).DifferentialsplicingofthreeexonsintheNR1-SubunitgeneratesuptoeightNR1-Subunitsplicevariantsand7ofthesehavebeenidentifiedinCDNAlibraries.Theseexonsencodea21aminoacidN-terminaldomain(N1)andadjacentsequencesintheC-terminus(C1andC2).SplicingouttheC2cassetteeliminatesthefirststopcodonandproducesanewreADIngframethatgeneratesanewsequenceof22aminoacids(C2′).ConsiderableattentionhasbeenfocusedonthedistributionandexpressionofthesesplicevariantsthatmayaffectthefunctionalpropertiesandregulationoftheNMDAR
Storage
25µgantibodyvial;lyophilizedfrom5mMammoniumbicarbonate.Theantibodyshouldbereconstitutedin50µlphosphatebufferedsaline(PBS:137mMNaCl,7.5mMNa2HPO4,2.7mMKCl,1.5mMKH2PO4,pH7.4)beforeuse.Afterreconstitutiontheantibodyshouldbealiquotedandstoredat-20°C.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
BradyML,DiazMR,IusoA,EverettJC,ValenzuelaCF,CaldwellKK(2013)ModerateprenatalalcoholexposurereducesplasticityandaltersNMDAreceptorsubunitcompositioninthedentategyrus.JNeurosci.33(3):1062-7.PMID:23325244
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik，他们在 Paul Greengard 博士的实验室共同工作，Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区，是一支敬业、友好、勤奋的科学家团队，致力于为您提供最优质的抗体和业内客户支持。
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