Rabbitpolyclonalantibody
PooledSerumTheantigenisapeptidefromtheNR1subunit,C2splicevariantinsertofratNMDAreceptor.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
TheionchannelsactivatedbyglutamatethataresensitivetoN-methyl-D-aspartate(NMDA)aredesignatedNMDAReceptors(NMDAR).TheNMDARplaysanessentialroleinmemory,neuronaldevelopmentandithasalsobeenimplicatedinseveraldisordersofthecentralnervoussystemincludingAlzheimer’s,epilepsyandischemicneuronalcelldeath(Grosshansetal.,2002;Wentholdetal.,2003;CarrollandZukin,2002).ThereareanumberofdifferentsplicevariantsoftheNR1subunit(Foldesetal.,1994;ZukinandBennett,1995).DifferentialsplicingofthreeexonsintheNR1subunitgeneratesuptoeightNR1subunitsplicevariantsand7ofthesehavebeenidentifiedinCDNAlibraries.Theseexonsencodea21aminoacidN-terminaldomain(N1)andadjacentsequencesintheC-terminus(C1andC2).SplicingouttheC2cassetteeliminatesthefirststopcodonandproducesanewreADIngframethatgeneratesanewsequenceof22aminoacids(C2′).ConsiderableattentionhasbeenfocusedonthedistributionandexpressionofthesesplicevariantsthatmayaffectthefunctionalpropertiesandregulationoftheNMDAR.
25µgantibodyvial;lyophilizedfrom5mMammoniumbicarbonate.Theantibodyshouldbereconstitutedin50µlphosphatebufferedsaline(PBS:137mMNaCl,7.5mMNa2HPO4,2.7mMKCl,1.5mMKH2PO4,pH7.4)beforeuse.Afterreconstitutiontheantibodyshouldbealiquotedandstoredat-20°C.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
BradyML,DiazMR,IusoA,EverettJC,ValenzuelaCF,CaldwellKK(2013)ModerateprenatalalcoholexposurereducesplasticityandaltersNMDAreceptorsubunitcompositioninthedentategyrus.JNeurosci.33(3):1062-7. PMID:23325244
MannieM.Y.Fan,HermanB.Fernandes,LilyY.J.Zhang,MichaelR.Hayden,andLynnA.Raymond(2007)AlteredNMDAReceptorTraffickinginaYeastArtificialChromosomeTransgenicMouseModelofHuntington’sDiseaseJ.Neurosci.,27:3768–3779. PMID:17409241
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。