Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Tyr1252oftheNR2BsubunitoftheratNMDAreceptor.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
TheNMDAreceptor(NMDAR)playsanessentialroleinmemory,neuronaldevelopmentandithasalsobeenimplicatedinseveraldisordersofthecentralnervoussystemincludingAlzheimer’s,epilepsyandischemicneuronalcelldeath(Grosshansetal.,2002;Wentholdetal.,2003;CarrollandZukin,2002).TheratNMDAR1(NR1)wasthefirstsubunitoftheNMDARtobecloned.TheNR1proteincanformNMDAactivatedchannelswhenexpressedinXenopusoocytesbutthecurrentsinsuchchannelsaremuchsmallerthanthoseseeninsitu.ChannelswithmorephysiologicalcharacteristicsareproducedwhentheNR1subunitiscombinedwithoneormoreoftheNMDAR2(NR2A-D)subunits(Ishiietal.,1993).PhosphorylationofTyr1252isthoughttopotentiateNMDAreceptor-dependentinfluxofcalcium(Takasuetal.,2002).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
TiannaR.Hicklin,PeterH.Wu,RichardA.Radcliffe,RonaldK.Freund,SusanM.Goebel-Goody,PauloR.Correa,WilliamR.Proctor,PaulJ.Lombroso,andMichaelD.Browning(2011)AlcoholinhibitionoftheNMDAreceptorfunction,long-termpotentiation,andfearlearningrequiresstriatal-enrichedproteintyrosinephosphatasePNAS,Apr2011;108:6650–6655.PMID:21464302
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。