Rabbitpolyclonalantibody

Size:

10µg

Formulation:

AffinityPurifiedfromPooledSerum

Specificity:

Rat,Mouse,Bovine,Canine

Applications:

WB1:1,000IHC1:1,000to1:2,000IP3µlper200µglysate

Species:

Rabbit

GeneName:

GRIN2B

MolecularReference:

~180kDa

CiteThisAntibody:

PhosphoSolutionsCat#1499-NR2B,RRID:AB_2492175

Storage

10µgantibodyvial;lyophilizedfrom5mMammoniumbicarbonate.Theantibodyshouldbereconstitutedin50µlphosphatebufferedsaline(PBS:137mMNaCl,7.5mMNa2HPO4,2.7mMKCl,1.5mMKH2PO4,pH7.4)beforeuse.Afterreconstitutiontheantibodyshouldbealiquotedandstoredat-20°C.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

ProductSpecificReferences

O’Leary,H.,Bernard,P.B.,Castano,A.M.,&Benke,T.A.(2016).EnhancedlongtermpotentiationanddecreasedAMPAreceptordesensitizationintheacuteperiodfollowingasinglekainateinducedearlylifeseizure.Neurobiologyofdisease,87,134-144.PMID:26706598

Bostrom,C.A.,Majaess,N.M.,Morch,K.,White,E.,&Christie,B.R.(2013).RescueofNMDAR-dependentsynapticplasticityinFmr1knock-outmice.Cerebralcortex,bht237.PMID:23968838

MeganL.Brady,MarvinR.Diaz,AnthonyIuso,JulieC.Everett,C.FernandoValenzuela,andKevinK.Caldwell.(2013)ModerateprenatalalcoholexposurereducesplasticityandaltersNMDAreceptorsubunitcompositioninthedentategyrus.JNeourosci.33(3):1062-7.PMID:23325244

TiannaR.Hicklin,PeterH.Wu,RichardA.Radcliffe,RonaldK.Freund,SusanM.Goebel-Goody,PauloR.Correa,WilliamR.Proctor,PaulJ.Lombroso,andMichaelD.Browning(2011)AlcoholinhibitionoftheNMDAreceptorfunction,long-termpotentiation,andfearlearningrequiresstriatal-enrichedproteintyrosinephosphatasePNAS,Apr2011;108:6650–6655.PMID:21464302

PradeepKurup,YongfangZhang,JianXu,DeepaV.Venkitaramani,VahramHaroutunian,PaulGreengard,AngusC.Nairn,andPaulJ.Lombroso(2010)A-beta-MediatedNMDAReceptorEndocytosisinAlzheimer’sDiseaseInvolvesUbiquitinationoftheTyrosinePhosphataseSTEP61.J.Neurosci.,30:5948–5957.PMID:20427654

JianXu,PradeepKurup,YongfangZhang,SusanM.Goebel-Goody,PeterH.Wu,AmmarH.Hawasli,MatthewL.Baum,JamesA.Bibb,andPaulJ.LombrosoExtrasynapticNMDAReceptorsCouplePreferentiallytoExcitotoxicityviaCalpain-MediatedCleavageofSTEP(2009)J.Neurosci.,29:9330–9343.PMID:19625523

KurtisD.Davies,SusanM.Goebel-Goody,StevenJ.Coultrap,andMichaelD.Browning(2008)LongTermSynapticDepressionThatIsAssociatedwithGluR1DephosphorylationbutNotalpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionicAcid(AMPA)ReceptorInternalizationJ.Biol.Chem.,283:33138–33146.PMID:18819923

ErinH.NorrisandSidneyStrickland(2007)ModulationofNR2B-regulatedcontextualfearinthehippocampusbythetissueplasminogenactivatorsystem.PNAS,104:13473-“13478.PMID:17673549

TatyanaChernova,JoernR.Steinert,ChristopherJ.Guerin,PierluigiNicotera,IanD.Forsythe,andAndrewG.Smith(2007)NeuriteDegenerationInducedbyHemeDeficiencyMediatedviaInhibitionofNMDAReceptor-DependentExtracellularSignal-RegulatedKinase1/2ActivationJ.Neurosci.,27:8475–8485.PMID:17687025

PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik，他们在 Paul Greengard 博士的实验室共同工作，Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区，是一支敬业、友好、勤奋的科学家团队，致力于为您提供最优质的抗体和业内客户支持。