Rabbitpolyclonalantibody
PooledSerumTheantigenisasyntheticphospho-peptidesurroundingthephosphoSer1244oftheNR2C-subunitofratNMDAreceptor.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
TheionchannelsactivatedbyglutamatethataresensitivetoN-methyl-Daspartate(NMDA)aredesignatedNMDAreceptors(NMDAR).TheNMDARplaysanessentialroleinmemory,neuronaldevelopmentandithasalsobeenimplicatedinseveraldisordersofthecentralnervoussystemincludingAlzheimer’s,epilepsyandischemicneuronalcelldeath(Grosshansetal.,2002;Wentholdetal.,2003;CarrollandZukin,2002).TheNMDAreceptorisalsooneoftheprincipalmoleculartargetsforalcoholintheCNS(Lovingeretal.,1989;Alvestadetal.,2003;Snelletal.,1996).TheNMDARisalsopotentiatedbyproteinphosphorylation(Luetal.,1999).TheNR2CsubunitofthereceptoristhoughttoinfluencetheNMDARconductancelevel(Ebralidzeetal.,1996).PhosphorylationofSer1244hasbeenshowntoregulateNMDAreceptorchannelfunction(Chenetal.,2006).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
AlvestadRM,GrosshansDR,CoultrapSJ,NakazawaT,YamamotoT,BrowningMD(2003)TyrosinedephosphorylationandethanolinhibitionofN-methyl-D-aspartatereceptorfunction.JBiolChem278:11020-11025.
CarrollRC,ZukinRS(2002)NMDA-receptortraffickingandtargeting:implicationsforsynaptictransmissionandplasticity.TrendsNeurosci25:571-577.
EbralidzeAK,RossiDJ,TonegawaS,SlaterNT(1996)ModificationofNMDAreceptorchannelsandsynaptictransmissionbytargeteddisruptionoftheNR2Cgene.JNeurosci16:5014-5025.
GrosshansDR,ClaytonDA,CoultrapSJ,BrowningMD(2002)LTPleadstorapidsurfaceexpressionofNMDAbutnotAMPAreceptorsinadultratCA1.NatNeurosci5:27-33.
LovingerDM,WhiteG,WeightFF(1989)EthanolinhibitsNMDA-activatedioncurrentinhippocampalneurons.Science243:1721-1724.
LuW-Y,XiongZ-G,LeiS,OrserBA,BrowningMD,MacDonaldJF(1999)G-proteincoupledreceptorsactviaproteinkinaseCandSrctoregulateNMDAreceptors.NatureNeurosci2:331-338.
SnellLD,NunleyKR,LickteigRL,BrowningMD,TabakoffB,HoffmanPL(1996)RegionalandsubunitspecificchangesinNMDAreceptormRNAandimmunoreactivityinmousebrainfollowingchronicethanolingestion.MolBrainRes40:71-78.
WentholdRJ,PrybylowskiK,StandleyS,SansN,PetraliaRS(2003)TraffickingofNMDAreceptors.AnnuRevPharmacolToxicol43:335-358.
ChenB,BraudS,BadgerJD,Isaac,JTR,Roche,KW(2006)RegulationofNR1/NR2CN-Methyl-D-aspartate(NMDA)receptorsbyphosphorylation.JBiolChem281:16583-16590.
Note:Dr.MichaelBrowning,aco-authoroffourofthecitedpapers,isPresidentandfounderofPhosphoSolutions.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。