Rabbitpolyclonalantibody
PooledSerumTheantigenisasyntheticpeptidecorrespondingtoaminoacidresiduesfromtheC-terminalregionofratTrkB.
TheantibodyispreparedfrompooledrabbitserumbyaffinitypurificationonacolumnmadewiththeC-terminalpeptideusedasantigen.
Tropomyosin-receptor-kinase(TRK)receptorsaremembersofthereceptortyrosinekinasefamilyandincludeTrkA,TrkBandTrkC.Eachofthesereceptortypeshasdifferentbindingaffinitiestotheneurotrophinsnervegrowthfactor(NGF),brain-derivedneurotrophicfactor(BDNF)andneurotrophin3(NT-3).TrkBhasthehighestaffinitytobindingBDNFwhichplayscriticalrolesinthefunctionandsurvivalofneuronsintheCNS(Soppetetal.,1991;Kleinetal.,1991).Additionally,alterationsinexpressionofTrkBhavebeenassociatedwithAlzheimer’sdisease(Ferreretal.,1999;Chenetal.,2008).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternblots.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
SoppetD,EscandonE,MaragosJ,MiddlemasDS,ReidSW,BlairJ,BurtonLE,StantonBR,KaplanDR,HunterT,NikolicsK,ParadaLF(1991)Theneurotrophicfactorsbrain-derivedneurotrophicfactorandneurotrophin-3areligandsforthetrkBtyrosinekinasereceptor.Cell.May31;65(5):895-903.
KleinR,NanduriV,JingSA,LamballeF,TapleyP,BryantS,Cordon-CardoC,JonesKR,ReichardtLF,BarbacidM(1991)ThetrkBtyrosineproteinkinaseisareceptorforbrain-derivedneurotrophicfactorandneurotrophin-3.Cell.Jul26;66(2):395-403.
FerrerI,MarínC,ReyMJ,RibaltaT,GoutanE,BlancoR,TolosaE,MartíE(1999)BDNFandfull-lengthandtruncatedTrkBexpressioninAlzheimerdisease.Implicationsintherapeuticstrategies.JNeuropatholExpNeurol.Jul;58(7):729-39.
ChenZ,SimmonsMS,PerryRT,WienerHW,HarrellLE,GoRC.(2008)Geneticassociationofneurotrophictyrosinekinasereceptortype2(NTRK2)WithAlzheimer’sdisease.AmJMedGenetBNeuropsychiatrGenet.Apr5;147(3):363-9.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。