Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser28ofp62.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
p62,alsoknownassequestosome1(SQSTM1),isashuttleproteintransportingpolyubiquitinatedproteinsforbothproteasomalandlysosomaldegradation.p62isanintegralcomponentofinclusionsinbrainsofvariousneurodegenerativedisorders,includingAlzheimerdisease(AD)neurofibrillarytangles(NFTs)andLewybodiesinParkinsondisease(Nogalaskaetal.,2009).p62playsanimportantroleintheprotectionofcellsfromthetoxicityofmisfoldedproteinsbyenhancingaggregateformationespeciallyinthelaterstages(Nakasoetal.,2004).PhosphorylationofSer28hasrecentlybeendemonstratedtoberelatedtothepathogenesisofParkinson’sdisease.
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
NogalskaA,TerraccianoC,D’AgostinoC,EngelWK,AskanasV(2009).p62/SQSTM1isoverexpressedandprominentlyaccumulatedininclusionsofsporADIcinclusion-bodymyositismusclefibers,andcanhelpdifferentiatingitfrompolymyositisanddermatomyositis.ActaNeuropathol.3:407-13.
NakasoK,YoshimotoY,NakanoT,TakeshimaT,FukuharaY,YasuiK,AragaS,YanagawaT,IshiiT,NakashimaK.(2004).Transcriptionalactivationofp62/A170/ZIPduringtheformationoftheaggregates:possIBLemechanismsandtheroleinLewybodyformationinParkinson’sdisease.BrainRes.1-2:42-51.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。