Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr269/Ser272ofhumanp62.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Theproteinscaffoldandsignalingregulatorp62(alsoknownassequestosome1(SQSTM1))isimportantincriticalcellularfunctions,includingbonehomeostasis,obesity,andcancer,becauseofitsinteractionswithvarioussignalingintermediaries.p62isoverexpressedinmanyhumancancersandisinducedduringcelltransformation.cdk1phosphorylatesp62invitroandinvivoatThr<sup>269</sup>andSer<sup>272</sup>,whichisnecessaryforthemaintenanceofappropriatecyclinB1levelsandthelevelsofcdk1activitynecessarytoallowcellstoproperlyenterandexitmitosis(Moscatetal.,2011).Thelackofcdk1-mediatedphosphorylationofp62leadstoafasterexitfrommitosis,translatingintoenhancedcellproliferationandtumOrigenesisinresponsetoRas-inducedtransformation(Moscatetal.,2011).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
MoscatJ,LinaresJF,AmanchyR,Diaz-MecoMT(2011)Phosphorylationofp62bycdk1ControlstheTimelyTransitofCellsthroughMitosisandTumorCellProliferation.MolCellBiol.1:105-17.
PankivS,LamarkT,BruunJA,ØvervatnA,BjørkøyG,andJohansen(2010).NucleocytoplasmicShuttlingofp62/SQSTM1andItsRoleinRecruitmentofNuclearPolyubiquitinatedProteinstoPromyelocyticLeukemiaBodies.JBiolChem.8:5941-53
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。