Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr402ofratp21activatedkinase2(PAK-2).ThepeptidesequenceusedisidenticalinPAK-1,2and3.Note:Thr402inPAK-2correspondstoThr423inhumanPAK-1.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Inmammals,thereareseveralidentifiedisoformsofp21-activatedproteinkinasesorPAKs:α-PAK(alsoknownasPAK-1)andβ-PAK(alsoknownasPAK-3)aremostlybrain-specific,while-PAK(alsoknownasPAK-2)isexpressedubiquitously(Jakobietal.,2003).MutationsofthegenecodingforPAK-3areassociatedwithX-linkedmentalretardationandrecentworkindicatesthatPAK-3isakeyregulatorofsynapseformationandplasticityinthehippocampus(Bodaetal.,2004).PAK-3isthoughttoplayakeyroleinregulationofcellshapeandmotilityaswellascelldeath(Jakobietal.,2003;Walteretal.,1998).AutophosphorylationofThr402intheproteinhasbeenfoundtobeessentialforactivationofPAK(Jakobietal.,2000).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Nie,J.,Lilley,B.N.,Pan,Y.A.,Faruque,O.,Liu,X.,Zhang,W.,Sanes,J.R.,Han,X.andShi,Y.,2013.SAD-Apotentiatesglucose-stimulatedinsulinsecretionasamediatorofglucagon-likepeptide1responseinpancreaticβcells.Molecularandcellularbiology,33(13),pp.2527-2534. PMID:23629625
JiaNie,ChaoSun,OmarFaruque,GuangmingYe,JiaLi,QiangrongLiang,ZhijieChang,WannianYang,XiaoHan,andYuguangShi(2012)SynapsesofAmphidsDefective(SAD-A)KinasePromotesGlucose-stimulatedInsulinSecretionthroughActivationofp21-activatedKinase(PAK1)inPancreaticβ-Cells.J.Biol.Chem.287:26435-26444.PMID:22669945
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。