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主营:神经科学领域的蛋白及抗体
℡ 4000-520-616
℡ 4000-520-616
PhosphoSolutions/PAK-1,2,3 (Thr402) Antibody/p187-402/100 µl
产品编号:p187-402
市  场 价:¥4574.70
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$345.00
品      牌: PhosphoSolutions
公      司:PhosphoSolutions
公司分类:
PhosphoSolutions/PAK-1,2,3 (Thr402) Antibody/p187-402/100 µl
商品介绍

Rabbitpolyclonalantibody

PooledSerum
Size:
100µl
Formulation:
AffinityPurifiedfromPooledSerum
Specificity:
Rat,Mouse,Human,Bovine,Canine
Applications:
WB1:1,000
Species:
Rabbit
MolecularReference:
~68kDato~70kDa
CiteThisAntibody:
PhosphoSolutionsCat#p187-402,RRID:AB_2492200
Antigen/Purification:ExpandCollapse

Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr402ofratp21activatedkinase2(PAK-2).ThepeptidesequenceusedisidenticalinPAK-1,2and3.Note:Thr402inPAK-2correspondstoThr423inhumanPAK-1.

Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.

BIOLOGicalSignificance:ExpandCollapse

Inmammals,thereareseveralidentifiedisoformsofp21-activatedproteinkinasesorPAKs:α-PAK(alsoknownasPAK-1)andβ-PAK(alsoknownasPAK-3)aremostlybrain-specific,while-PAK(alsoknownasPAK-2)isexpressedubiquitously(Jakobietal.,2003).MutationsofthegenecodingforPAK-3areassociatedwithX-linkedmentalretardationandrecentworkindicatesthatPAK-3isakeyregulatorofsynapseformationandplasticityinthehippocampus(Bodaetal.,2004).PAK-3isthoughttoplayakeyroleinregulationofcellshapeandmotilityaswellascelldeath(Jakobietal.,2003;Walteretal.,1998).AutophosphorylationofThr402intheproteinhasbeenfoundtobeessentialforactivationofPAK(Jakobietal.,2000).

Storage

100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

ProductSpecificReferences

Nie,J.,Lilley,B.N.,Pan,Y.A.,Faruque,O.,Liu,X.,Zhang,W.,Sanes,J.R.,Han,X.andShi,Y.,2013.SAD-Apotentiatesglucose-stimulatedinsulinsecretionasamediatorofglucagon-likepeptide1responseinpancreaticβcells.Molecularandcellularbiology,33(13),pp.2527-2534. PMID:23629625

JiaNie,ChaoSun,OmarFaruque,GuangmingYe,JiaLi,QiangrongLiang,ZhijieChang,WannianYang,XiaoHan,andYuguangShi(2012)SynapsesofAmphidsDefective(SAD-A)KinasePromotesGlucose-stimulatedInsulinSecretionthroughActivationofp21-activatedKinase(PAK1)inPancreaticβ-Cells.J.Biol.Chem.287:26435-26444.PMID:22669945

品牌介绍

PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。

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